Application of low intensity ultrasound to enhance the activity of anammox microbial consortium for nitrogen removal
Application of low intensity ultrasound to enhance the activity of anammox microbial consortium for nitrogen removal
复制标题
应用低强度超声增强厌氧氨氧化微生物群落脱氮活性
DOI：
10.1016/j.biortech.2010.12.050
复制
发表时间：
2011-03-01
影响因子：
11.4
通讯作者：
Wei, Haifeng
中科院分区：
文献类型：
--
作者：
Duan, Xiumei;Zhou, Jiti;Wei, Haifeng
本研究通过相同辐照频率25 kHz的批量实验，研究了低强度超声对厌氧氨氧化微生物群落脱氮活性的影响。当超声强度为0.3 w cm(-2)、最佳照射时间为4 min时,总氮去除率提高了约25.5%,且进一步实验证明这种效果可以持续约6 d。细胞外聚合物分析表明，超声后第一天碳水化合物、蛋白质和总细胞外物质增加最大，分别为28.8%、30.5%和29.7%。随着时间的延长，细胞外碳水化合物、蛋白质的生成率逐渐下降。透射电镜观察表明，超声处理的厌氧氨氧化微生物群落的细胞壁变薄，导致细胞外物质的释放增加。结果表明，低强度超声波的应用可以增强厌氧氨氧化微生物群落的活性，并最终增强脱氮的潜力。 (C) 2010 Elsevier Ltd. 保留所有权利。
In this study, effect of low intensity ultrasound on the activity of anammox microbial consortium for nitrogen removal was investigated through batch experiments at the same irradiation frequency of 25 kHz. Total nitrogen removal rate increased by about 25.5% when ultrasound intensity of 0.3 w cm(-2) was applied at an optimal irradiation time of 4 min, and further experiments demonstrated that this effect could last for about 6 days. Analysis of extracellular polymeric substances indicated that the maximum increase of carbohydrate, protein and total extracellular substances was obtained on the first day after ultrasound, which was 28.8%, 30.5% and 29.7%, respectively. As the time prolonged, the production rate of extracellular carbohydrate, protein decreased gradually. Transmission electron microscopy observation demonstrated that ultrasounded cell wall of anammox microbial consortium became thinner resulting in increased release of extracellular substances. The results suggested that application of low intensity ultrasound may enhance the activity of anammox microbial consortium and ultimately the potential for nitrogen removal. (C) 2010 Elsevier Ltd. All rights reserved.