Development of a Cherenkov light imaging system for studying the dynamics of radiocesium in plants
Development of a Cherenkov light imaging system for studying the dynamics of radiocesium in plants
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DOI：
10.1080/00223131.2017.1299051
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发表时间：
2017-01-01
影响因子：
1.2
通讯作者：
Kawachi, Naoki
中科院分区：
文献类型：
--
作者：
Kurita, Keisuke;Suzui, Nobuo;Kawachi, Naoki
放射性铯（Cs-137）分布的高分辨率图像是研究植物中铯动力学所必需的。切伦科夫光成像系统可以使用光学相机可视化发射β粒子的放射性核素的精细分布。为了评价系统的线性，用放射性为10-2000 kBq的Cs-137点源进行成像测试。结果表明，该系统的成像强度与Cs-137的放射性活度之间具有良好的线性关系。我们开发了一种植物成像系统，利用该系统研究放射性铯在完整植物中的运动。为了证明在植物中成像放射性铯的能力，用完整的大豆植物进行了为期四天的实验。将11天龄的大豆植物的根浸入20 mL含有放射性为10 MBq的Cs-137的培养液中，不含钾。一天后，用含钾但不含Cs-137的溶液替换该溶液。大豆植株在该系统中处于健康状态，高分辨率序列图像显示Cs-137被运输到地上部并在节中积累。因此，切伦科夫光成像是有前途的完整的植物成像放射性铯。
High-resolution images of radiocesium (Cs-137) distribution are required to study cesium kinetics in plants. A Cherenkov light imaging system can visualize fine distributions of radionuclides emitting beta particles using an optical camera. To evaluate the linearity of the system, an imaging test was performed with point sources of Cs-137, with a radioactivity of 10-2000 kBq. The results indicated that the system has a good linearity between the image intensity and the radioactivity of Cs-137. We developed an imaging system for plants using this system to study radiocesium movement in intact plants. To demonstrate the ability to image radiocesium in a plant, an experiment was performed with an intact soybean plant for four days. The root of an 11-day-old soybean plant was dipped in 20 mL of a culture solution containing Cs-137 with a radioactivity of 10 MBq without potassium. After one day, the solution was replaced with one with potassium but no Cs-137. The soybean plant was in healthy condition in the system, and the high-resolution serial images indicated that Cs-137 was transported to the shoot and accumulated in the node. Therefore, Cherenkov light imaging is promising for imaging radiocesium in intact plants.