The chorionic gonadotropin alpha-subunit gene is on human chromosome 18 in JEG cells.
The chorionic gonadotropin alpha-subunit gene is on human chromosome 18 in JEG cells.
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绒毛膜促性腺激素 α 亚基基因位于 JEG 细胞中的人类 18 号染色体上。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
P. Kohler
中科院分区：
文献类型：
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作者：
J. Hardin;M. Riser;J. Trent;P. Kohler
人绒毛膜促性腺激素（hCG）α亚单位的基因已被暂时分配给人类18号染色体。该定位通过使用Southern印迹分析完成。利用hCG α亚基的全长cDNA探针和从小鼠和人细胞之间的一系列体细胞杂种中分离的DNA进行分配。此外，原位杂交与正常人外周血淋巴细胞作为人类染色体的来源，并与相同的cDNA探针证实了这一结果。人18号染色体的存在是检测α-hCG基因特征性DNA片段所必需的。这些结果与我们先前的观察一致，即人类染色体10和18是培养细胞中hCG产生所必需的。
The gene for the alpha subunit of human chorionic gonadotropin (hCG) has been tentatively assigned to human chromosome 18. This localization was accomplished through the use of Southern blot analysis. A full-length cDNA probe for the hCG alpha subunit and DNA isolated from a series of somatic hybrids between mouse and human cells were utilized to make this assignment. In addition, in situ hybridization with normal human peripheral blood lymphocytes as a source of human chromosomes and with the same cDNA probe confirmed this result. The presence of human chromosome 18 was required for the detection of DNA fragments characteristic of the alpha-hCG gene. These results are consistent with our previous observation that human chromosomes 10 and 18 are required for the production of hCG in cultured cells.