Transport of Intensity phase imaging by intensity spectrum fitting of exponentially spaced defocus planes.
Transport of Intensity phase imaging by intensity spectrum fitting of exponentially spaced defocus planes.
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DOI：
10.1364/oe.22.010661
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发表时间：
2014-05
期刊：
影响因子：
3.8
通讯作者：
Jingshan Zhong;Rene A. Claus;J. Dauwels;L. Tian;L. Waller
中科院分区：
文献类型：
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作者：
Jingshan Zhong;Rene A. Claus;J. Dauwels;L. Tian;L. Waller
我们提出了一种替代方法来解决运输的强度方程（TIE）从一个堆栈的通过聚焦强度的显微镜或无透镜成像仪所采取的图像。我们的方法能够实现定量相位和幅度成像，提高了精度，减少了数据捕获，同时还具有计算效率和抗噪声能力。我们使用强度如何随空间频率域中的传播而变化的先验知识，以约束用于估计轴向强度导数的拟合算法[高斯过程（GP）回归]。在频域中解决该问题激发了一种有效的测量方案，该方案以指数间隔的焦点步长捕获图像，从而显著减少了所需图像的数量。困扰传统TIE方法的低频伪影可以被抑制，而无需过多的捕获图像。我们通过在明场显微镜下恢复人类脸颊细胞的相位来验证我们的技术。
We propose an alternative method for solving the Transport of Intensity equation (TIE) from a stack of through-focus intensity images taken by a microscope or lensless imager. Our method enables quantitative phase and amplitude imaging with improved accuracy and reduced data capture, while also being computationally efficient and robust to noise. We use prior knowledge of how intensity varies with propagation in the spatial frequency domain in order to constrain a fitting algorithm [Gaussian process (GP) regression] for estimating the axial intensity derivative. Solving the problem in the frequency domain inspires an efficient measurement scheme which captures images at exponentially spaced focal steps, significantly reducing the number of images required. Low-frequency artifacts that plague traditional TIE methods can be suppressed without an excessive number of captured images. We validate our technique experimentally by recovering the phase of human cheek cells in a brightfield microscope.