Tr-KIT/c-KIT ratio in renal cell carcinoma
Tr-KIT/c-KIT ratio in renal cell carcinoma
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DOI：
10.1007/s11033-019-04985-3
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发表时间：
2019-10-01
影响因子：
2.8
通讯作者：
Abur, Ummet
中科院分区：
文献类型：
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作者：
Ergun, Sercan;Altay, Diler Us;Abur, Ummet
截短试剂盒(tr-kit)是c-kit蛋白的一种替代变体。以前的研究已经清楚地证明，c-kit与肾癌的各种致癌过程有关。然而，RT-KIT在肾癌发生发展中的生物学意义仍不清楚。因此，本研究旨在探讨肾细胞癌与被认为激活某些致癌途径的TR-KIT之间的可能联系。本研究利用肾癌基因芯片对9例正常人正常肾组织和10例1期、5例2期、13例3期和11例4期肾癌患者的肾癌组织进行了基因表达谱分析。采用实时荧光定量聚合酶链式反应(Real-Time-PCR，RT-PCR)检测tr-kit/c-kit的表达。比较肿瘤组织和正常肾组织中TRKIT/c-KIT的表达比例，并与肾癌患者的临床参数进行相关分析。在肿瘤组织中，TR-KIT/c-KIT的表达比率约为对照组的4倍(p=0.001)。此外，在Fuhrman核2、3和4级中，TR-KIT/c-KIT的表达比率分别约为正常的2、3和6倍(p=0.009)。此外，透明细胞肾癌和乳头状肾细胞癌的tr-kit/c-kit表达水平显著高于嫌色肾癌(p=0.016)。在本研究中，首次发现肾细胞癌组织中tr-kit/c-kit的表达上调，且tr-kit/c-kit的高表达与肾癌的临床特征和预后不良有关。我们的结果提示，tr-kit/c-kit表达比值的升高可能是肾癌患者预后的一个有用的指标。
Truncated KIT (tr-KIT) is an alternative variant of c-KIT protein. Previous studies have clearly documented that c-KIT was associated with various oncogenic processes in RCC. However, the biological significance of tr-KIT in RCC development and progression remains unclear. So, it was aimed to investigate the possible association between RCC and tr-KIT which is thought to activate some oncogenic pathways. In this study, Kidney Cancer cDNA Array containing a total of 48 cDNA samples from the normal kidney tissues of 9 healthy subjects and kidney tumor tissues of 10 stage-1, 5 stage-2, 13 stage-3 and 11 stage-4 RCC patients was used for gene expression analysis. Real-Time PCR method was used to measure tr-KIT/c-KIT expression ratios. tr-KIT/c-KIT expression ratio was compared between tumor and normal samples, and statistically correlated with the clinical parameters of RCC patients. tr-KIT/c-KIT expression ratio was approximately 4-times higher in tumor samples than control ones (p = 0.001). Also, tr-KIT/c-KIT expression ratio was approximately two, three and six times higher in Fuhrman nuclear grades 2, 3 and 4 than normal, respectively (p = 0.009). Moreover, clear cell and papillary RCC has a significantly higher level of tr-KIT/c-KIT expression ratio than chromophobe RCC (p = 0.016). In the current study, it was stated for the first time that tr-KIT/c-KIT expression ratio was up-regulated in RCC tissues, and high tr-KIT/c-KIT expression ratio was correlated with more aggressive clinical features and poor patient prognosis. Our results suggest that increased tr-KIT/c-KIT expression ratio might be useful as a prognostic marker for RCC patients.