Zearalenone and its metabolites in the tissues of female wild boars exposed per os to mycotoxins
Zearalenone and its metabolites in the tissues of female wild boars exposed per os to mycotoxins
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DOI：
10.1016/j.toxicon.2016.02.012
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发表时间：
2016-05-01
期刊：
影响因子：
2.8
通讯作者：
Nicpon, Jozef
中科院分区：
文献类型：
--
作者：
Gajecka, Magdalena;Slawuta, Piotr;Nicpon, Jozef
该研究在18只临床健康的雌性野猪中进行，初始体重为35 +/- 2 kg。将动物分为两个实验组（I组和II组）和一个对照组（C组），每组6只雌性野猪。第I组动物每两个月口服纯玉米赤霉烯酮（ZEN）150 μ g/kg BW，连续7天，而第II组动物接受自然污染的饲料50 μ g/kg BW/天。雌性野猪暴露于ZEN超过1年。对照组动物喂食安慰剂。在实验最后一天屠宰后3 min内采集组织样品（背肌、肝左叶、左肾、脾、心肌顶端部分、肺前叶、左卵巢、子宫左角中央部分）。在第I组中，脾脏（19.813 ng/g）、心肌（18.105 ng/g）和肾脏（14.555 ng/g）中的ZEN水平最高。在第II组中，在肌肉组织（12.033 ng/g）、子宫（10.821 ng/g）和肾脏（10.463 ng/g）中观察到ZEN的最高浓度。在相同组织中观察到残留因子的最高值。在检查的雌性野猪中，经口暴露于母体物质的天然来源或ZEN及其代谢产物的组合导致分析组织中ZEN的浓度不同。在两个实验组中，玉米赤霉烯酮浓度与CF值相容。(C)2016爱思唯尔有限公司版权所有。
The study was performed on 18 clinically healthy female wild boars with initial body weight of 35 +/- 2 kg. The animals were divided into two experimental groups (group I and group II) and one control group (group C) of 6 female wild boars per group. Group I animals were administered per os pure zearalenone (ZEN) at 150 mu g/kg BW every two months for 7 subsequent days, whereas group II animals received feed naturally contaminated with ZEN at 50 mu g/kg BW/day. Female wild boars were exposed to ZEN over a period of 1 year. Control group animals were fed a placebo. Tissue samples (dorsal muscles, left lobe of liver, left kidney, spleen, apical part of the cardiac muscle, cranial lobe of lung, left ovary, central part of the left horn of the uterus) were collected on the last day of the experiment within 3 min after slaughter. In group I, the highest ZEN levels were noted in the spleen (19.813 ng/g), cardiac muscle (18.105 ng/g) and kidneys (14.555 ng/g). In group II, the highest concentrations of ZEN were observed in muscle tissue (12.033 ng/g), uterus (10.821 ng/g) and kidneys (10.463 ng/g). The highest values of the carry-over factor were noted in the same tissues. In the examined female wild boars, per os exposure to natural sources of the parent substance or a combination of ZEN and its metabolites led to different concentrations of ZEN in the analyzed tissues. Zearalenone concentrations were compatible with CF values in both experimental groups. (C) 2016 Elsevier Ltd. All rights reserved.