Depletion of intracellular polyamines may alter DNA conformation in 9L rat brain tumor cells.
Depletion of intracellular polyamines may alter DNA conformation in 9L rat brain tumor cells.
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细胞内多胺的消耗可能会改变 9L 大鼠脑肿瘤细胞中的 DNA 构象。
DOI：
10.1126/science.6408733
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Shafer,RH
中科院分区：
文献类型：
--
作者：
Hung,DT;Marton,LJ;Deen,DF;Shafer,RH
用粘弹测定法测定，用α-二氟甲基鸟氨酸处理9 L大鼠脑肿瘤细胞，使其多胺耗竭，DNA构象发生显著改变。减少细胞内腐胺和亚精胺的浓度，其浓度在控制细胞中的5%以下的9 L细胞DNA的敏感性降低x-照射，并增加了最大的粘弹性阻滞时间的DNA。这两种现象都被逆转，通过添加外源腐胺。
Depletion of polyamines in 9L rat brain tumor cells by treatment with α-difluoromethylornithine dramatically altered DNA conformation as measured by viscoelastometry. The reduction of intracellular putrescine and spermidine concentrations to less than 5 percent of their concentrations in control cells decreased the sensitivity of 9L cell DNA to x-irradiation and increased the maximum viscoelastic retardation time of the DNA. Both of these phenomena were reversed by addition of exogenous putrescine.