Selenium protection against mercury-induced apoptosis and growth inhibition in cultured K-562 cells
Selenium protection against mercury-induced apoptosis and growth inhibition in cultured K-562 cells
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DOI：
10.1385/bter:92:2:105
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发表时间：
2003-05-01
影响因子：
3.9
通讯作者：
Lindh, U
中科院分区：
文献类型：
--
作者：
Frisk, P;Wester, K;Lindh, U
研究了硒和氯化汞 (MC) 相互作用对细胞生长和细胞死亡的影响。将人 K-562 细胞用亚硒酸盐（5 或 50 PM）或硒代蛋氨酸（10 或 50 muM）以及 MC（35 或 50 muM）同时进行预处理或同时处理。 35-muM MC 处理明显抑制细胞生长，汞处理和汞硒处理的细胞之间没有明显差异。此外，对于用 35 muM MC 进行的所有硒处理，在所有观察结果中，细胞凋亡频率相似。在同时处理的亚硒酸盐和 50-muM MC 组合中，在暴露 48 小时和 96 小时时，通过增加细胞生长和降低细胞凋亡频率，显示出亚硒酸盐依赖性保护。两种硒代蛋氨酸处理都显示出保护作用，观察到在暴露 48 小时和 96 小时时细胞生长增加，并且在暴露 96 小时时细胞凋亡频率降低。
Selenium and mercuric chloride (MC) interactions regarding effects on cell growth and cell death have been studied. Human K-562 cells were pretreated or simultaneously treated with either selenite (5 or 50 PM) or selenomethionine (10 or 50 muM) and with MC (35 or 50 muM). The 35-muM MC treatments resulted in a clear inhibition of cell growth with no obvious difference between mercury-treated and mercury-selenium-treated cells. Furthermore, the apoptotic frequency was similar at all observations for all selenium treatments with 35 muM MC. In the simultaneously treated selenite and 50-muM MC combinations, a selenite-dependent protection was shown both by increased cell growth and by lower apoptotic frequency at 48 and 96 h of exposure. Both treatments with selenomethionine showed protection observed as an increased cell growth at 48 and 96 h and as decreased apoptotic frequency at 96 h of exposure.