Does Sequence Type 33 of Shiga Toxin-Producing Escherichia coli O91 Cause Only Mild Symptoms?
Does Sequence Type 33 of Shiga Toxin-Producing Escherichia coli O91 Cause Only Mild Symptoms?
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产志贺毒素大肠杆菌 O91 的序列类型 33 是否仅引起轻微症状？
DOI：
10.1128/jcm.02335-14
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Fujimoto S
中科院分区：
文献类型：
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作者：
Maeda E;Murakami K;Etoh Y;Onozuka D;Sera N;Asoshima N;Honda M;Narimatsu H;Iyoda S;Watahiki M;Fujimoto S
欧洲国家。STEC O91通常是从法国的散发性成人溶血性尿毒症综合征(HUS)病例中分离出来的(1例)，而在德国，序列类型为442(ST442)的STEC O91偶尔会引起HUS(2)。在日本，STECO91是一种主要的非O157STEC O血清群，主要从无症状携带者中分离出来(有关监测数据，请参阅http://www.美国国立卫生研究院。去。Jp/niid/en/typhi-m/iasr-reference/230-iasr-data/2972-iasr-table-be-p.超文本标记语言[2014年9月10日访问])。然而，它偶尔会出现在没有HUS的轻微症状的患者中，如腹痛和水样腹泻(3)。这种肠道病原体在日本和欧洲国家的毒力差异的原因尚不清楚。梅尔曼等人。用多位点序列分型(MLST)分析STEC O91亚型分离株，报道了所有ST33分离株和所有单基因座变异株都可以分型为O91：H14，O91：静止，或O91：不能分型，在人类中表现出相对较低的致病性(无症状感染或引起水样腹泻、腹痛、发热或出血性大便，没有HUS)(2)。我们通过MLST、脉冲场凝胶电泳(PFGE)、抗菌素敏感性试验和毒力相关基因检测对50株STEC O91分离株进行了鉴定。从水样腹泻和腹痛但无HUS、发热或血便的患者中分离到6株，从无症状携带者中获得44株。在这项研究中调查的48个菌株占所有菌株的23%
European countries. STEC O91 is often isolated from sporadic adult cases of hemolytic-uremic syndrome (HUS) in France (1), while in Germany, STEC O91 with sequence type 442 (ST442) occasionally causes HUS (2). In Japan, STEC O91 is a major non-O157 STEC O serogroup, and it is isolated mainly from asymptomatic carriers (for surveillance data, see http://www. nih. go. jp/niid/en/typhi-m/iasr-reference/230-iasr-data/2972-iasr-table-be-p. html [accessed 10 September 2014]). However, it is occasionally found in patients with minor symptoms, such as abdominal pain and watery diarrhea without HUS (3). The reasons for the differences in virulence of this enteropathogen between Japan and European countries remain unknown. Mellmann et al. subtyped STEC O91 isolates using multilocus sequence typing (MLST) analysis and reported that all O91 isolates of ST33, and all single-locus variants, could be serotyped as O91: H14, O91: nonmotile, or O91: untypeable and demonstrated relatively low pathogenicity in humans (asymptomatic infection or causing watery diarrhea, abdominal pain, fever, or bloody stool without HUS)(2).We characterized 50 STEC O91 isolates from humans in Japan by MLST, pulsed-field gel electrophoresis (PFGE), antimicrobial susceptibility testing, and virulence-related gene detection. Six isolates were obtained from patients with watery diarrhea and abdominal pain but without HUS, fever, or bloody stool, and 44 isolates were obtained from asymptomatic carriers. Forty-eight of the isolates investigated in this study accounted for 23% of all