Osmotic gradients induce stable dome morphogenesis on extracellular matrix
Osmotic gradients induce stable dome morphogenesis on extracellular matrix
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DOI：
10.1242/jcs.243865
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发表时间：
2020-07-01
影响因子：
4
通讯作者：
Haga, Hisashi
中科院分区：
文献类型：
--
作者：
Ishida-Ishihara, Sumire;Akiyama, Masakazu;Haga, Hisashi
形态发生的一个基本过程是穹窿的形成，但许多涉及的机制尚未被探索。先前的体外研究表明，渗透梯度是圆顶形成的驱动因素。然而，这些研究是在没有细胞外基质（ECM）的情况下进行的，而细胞外基质为形态发生提供了结构支持。使用ECM，我们观察到基础高渗应激在体外诱导稳定的圆顶，这在以前的研究中没有见过。这些圆顶的形成是ECM通过水通道蛋白的水运输活动而膨胀的结果。基于计算机模拟，在细胞拉伸和增强的水分输送之间的正反馈作用下，不均匀膨胀是圆顶形成的原因。这些结果表明，渗透梯度通过增强水运活动和随后的ECM肿胀诱导穹窿形态发生。
One of the fundamental processes in morphogenesis is dome formation, but many of the mechanisms involved are unexplored. Previous in vitro studies showed that an osmotic gradient is the driving factor of dome formation. However, these investigations were performed without extracellular matrix (ECM), which provides structural support to morphogenesis. With the use of ECM, we observed that basal hypertonic stress induced stable domes in vitro that have not been seen in previous studies. These domes developed as a result of ECM swelling via aquaporin water transport activity. Based on computer simulation, uneven swelling, with a positive feedback between cell stretching and enhanced water transport, was a cause of dome formation. These results indicate that osmotic gradients induce dome morphogenesis via both enhanced water transport activity and subsequent ECM swelling.