Pilot-scale purification of zeamatin, an antifungal protein from maize.
Pilot-scale purification of zeamatin, an antifungal protein from maize.
复制标题
玉米素（一种来自玉米的抗真菌蛋白）的中试规模纯化。
DOI：
10.1021/bp9901365
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Selitrennikoff,CP
中科院分区：
文献类型：
--
作者：
Wilson,S;Mahiou,B;Reiger,R;Tentler,S;Schimoler,R;Orndorff,S;Selitrennikoff,CP
Zeamatin is an antifungal protein isolated from the seeds ofZea mays. A practical process for isolation and purification was developed to increase recovery yields for future testing of zeamatin as a novel therapeutic drug. Zeamatin was extracted with buffer from corn meal milled to flour. Solids were removed from the extract using pressure filtration, and zeamatin was purified using two separate reverse‐phase chromatography steps. Determination of the amino‐terminal amino acid sequence, Western blotting using anti‐zeamatin antibody, and activity againstCandida albicanswere used to confirm that the purified protein was zeamatin. From 5 kg of corn meal, ∼110 mg of zeamatin was purified to apparent homogeneity.
