Gap junction intercellular communication during lymphocyte transendothelial migration
Gap junction intercellular communication during lymphocyte transendothelial migration
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DOI：
10.1006/cbir.2001.0840
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发表时间：
2002-01-01
影响因子：
3.9
通讯作者：
Evans, WH
中科院分区：
文献类型：
--
作者：
Oviedo-Orta, E;Errington, RJ;Evans, WH
淋巴细胞穿过中央或外周组织内皮的迁移是激活或分化后发生的过程，涉及以粘附和异型信号“串扰”为特征的细胞间相互作用。由于淋巴细胞和内皮细胞表达连接蛋白（缝隙连接细胞间通道的亚基蛋白），我们研究了这些通道在淋巴细胞跨内皮迁移过程中是否具有异型信号传导特征。我们使用 FACS 分析表明，钙黄绿素（一种间隙连接渗透性荧光染料）从内皮细胞层转移到淋巴细胞。研究表明间隙连接参与细胞间染料转移，该过程受到连接蛋白模拟肽的抑制，连接蛋白模拟肽是一类新型试剂，可阻断间隙连接通讯。通过双光子激光扫描显微镜获得了淋巴细胞间隙连接参与跨内皮迁移过程中细胞间通讯的进一步证据。尽管间隙连接通讯被连接蛋白模拟肽抑制，但它们对轮回过程影响不大。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
Migration of lymphocytes across the endothelium of central or peripheral tissues, a process occurring following activation or differentiation, involves cell to cell interactions featuring adhesion and heterotypic signalling 'cross-talk'. Since lymphocytes and endothelial cells express connexins, the subunit proteins of gap junction intercellular channels, we investigated whether these channels feature in heterotypic signalling during transendothelial migration of lymphocytes. We show, using FACS analysis, that calcein, a gap junction permeant fluorescent dye, was transferred from endothelial cell layers to lymphocytes. The gap junction involvement in intercellular dye transfer was reinforced by studies showing that the process was inhibited by connexin mimetic peptides, a new class of reagents shown to block gap junction communication. Further evidence for the involvement of lymphocyte gap junctions in intercellular communication during transendothelial migration was obtained by two-photon laser scanning microscopy. Although gap junctional communication was inhibited by connexin mimetic peptides, they had little influence on the transmigration process. (C) 2002 Elsevier Science Ltd. All rights reserved.