Stable, biocompatible lipid vesicle generation by solvent extraction-based droplet microfluidics
Stable, biocompatible lipid vesicle generation by solvent extraction-based droplet microfluidics
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DOI：
10.1063/1.3665221
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发表时间：
2011-12-01
期刊：
影响因子：
3.2
通讯作者：
Lee, Abraham P.
中科院分区：
文献类型：
--
作者：
Teh, Shia-Yen;Khnouf, Ruba;Lee, Abraham P.
在本文中，我们提出了一个微流控平台的稳定，单分散的脂质囊泡直径20-110 μ m的连续生成。我们的方法利用微流体流动聚焦液滴生成设计，通过改变系统的流体流速来控制囊泡的大小，以生成具有窄尺寸分布的囊泡。双重乳液首先在连续的流动聚焦通道几何形状中产生，然后通过受控的溶剂萃取过程形成脂质膜。由于在该过程中没有使用强溶剂，因此我们的方法允许安全封装和操作各种生物实体，包括细胞，蛋白质和核酸。通过该方法产生的囊泡是稳定的，并且具有至少3个月的保质期。在这里，我们展示了在脂质囊泡内的蛋白质的无细胞体外合成作为人工细胞发展的第一步。(C)2011年美国物理学会。[doi：10.1063/1.3665221]
In this paper, we present a microfluidic platform for the continuous generation of stable, monodisperse lipid vesicles 20-110 mu m in diameter. Our approach utilizes a microfluidic flow-focusing droplet generation design to control the vesicle size by altering the system's fluid flow rates to generate vesicles with narrow size distribution. Double emulsions are first produced in consecutive flow-focusing channel geometries and lipid membranes are then formed through a controlled solvent extraction process. Since no strong solvents are used in the process, our method allows for the safe encapsulation and manipulation of an assortment of biological entities, including cells, proteins, and nucleic acids. The vesicles generated by this method are stable and have a shelf life of at least 3 months. Here, we demonstrate the cell-free in vitro synthesis of proteins within lipid vesicles as an initial step towards the development of an artificial cell. (C) 2011 American Institute of Physics. [doi:10.1063/1.3665221]