Stimulation of serglycin and CD44 mRNA expression in endothelial cells exposed to TNF-α and IL-1α
Stimulation of serglycin and CD44 mRNA expression in endothelial cells exposed to TNF-α and IL-1α
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DOI：
10.1016/s0304-4165(99)00096-3
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发表时间：
1999-08-05
影响因子：
3
通讯作者：
Ranheim, T
中科院分区：
文献类型：
--
作者：
Kulseth, MA;Kolset, SO;Ranheim, T
丝甘肽是一种广泛分布的蛋白多糖，以前认为是造血细胞特异性的。然而，所呈现的结果表明丝甘肽mRNA在造血细胞系统外表达。在内皮细胞和平滑肌细胞中检测到高水平的丝甘肽mRNA，而在皮肤成纤维细胞中检测到低水平。为了进一步分析丝甘肽在内皮细胞中的重要性，在通过促炎细胞因子TNF-α和IL-1 α活化源自人脐带静脉的内皮细胞系（HUV-EC-C）后测量丝甘肽mRNA的表达。丝甘素mRNA水平呈时间和剂量依赖性增加。TNF-α（7 ng/ml）是最有效的诱导剂，刺激24小时后，sergylycin mRNA水平增加2.5倍。丝甘肽已被证明是CD 44的配体，CD 44是一种在内皮细胞中表达的膜蛋白。刺激内皮细胞后，CD 44 mRNA水平也增加。同样，TNF-α（7 ng/ml）被证明是最有效的诱导剂，刺激24小时后，CD 44 mRNA水平增加5.5倍。内皮细胞的TNF-α和IL-1 α刺激均导致[S-35]硫酸盐掺入大分子的总量增加，这可能表明蛋白聚糖的总产量增加。促炎剂对内皮细胞的刺激导致sergylycin和CD 44 mRNA表达的增加，表明sergylycin以及CD 44可能参与白细胞迁移的炎症过程。(C)1999 Elsevier Science B. V.保留所有权利。
Serglycin is a widely distributed proteoglycan, previously assumed to be hematopoietic cell specific. However, the results presented show that serglycin mRNA is expressed outside the hematopoietic cell system. High levels of serglycin mRNA were detected in endothelial cells and smooth muscle cells, whereas low levels were detected in skin fibroblasts. To further analyze the importance of serglycin in endothelial cells, the expression of serglycin mRNA was measured following activation of an endothelial cell line derived from human umbilical cord vein (HUV-EC-C), by the proinflammatory cytokines TNF-alpha and IL-1 alpha. The level of serglycin mRNA increased in a time- and dose-dependent way. TNF-alpha (7 ng/ml) was the most potent inducer, increasing the level of serglycin mRNA 2.5 times after 24 h of stimulation. Serglycin has been shown to be a ligand for CD44, a membrane protein expressed in endothelial cells. Following stimulation of the endothelial cells, the level of CD44 mRNA also increased. Again, TNF-alpha (7 ng/ml) turned out to be the most potent inducer, increasing the level of CD44 mRNA 5.5 times after 24 h of stimulation. Both TNF-alpha and IL-1 alpha stimulation of the endothelial cells resulted in an increase in the total incorporation of [S-35]sulfate into macromolecules, which probably indicates an increase in the total production of proteoglycans. A stimulation of endothelial cells by proinflammatory agents resulted in an increase in both serglycin and CD44 mRNA expression, indicating that serglycin, as well as CD44, may participate in the inflammatory process of leukocyte migration. (C) 1999 Elsevier Science B.V. All rights reserved.