Anthocyanin profile and antioxidant capacity of black carrots (Daucus carota L. ssp sativus var. atrorubens Alef.) from Cuevas Bajas, Spain
Anthocyanin profile and antioxidant capacity of black carrots (Daucus carota L. ssp sativus var. atrorubens Alef.) from Cuevas Bajas, Spain
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DOI：
10.1016/j.jfca.2013.11.005
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发表时间：
2014-02-01
影响因子：
4.3
通讯作者：
Casado, Juan
中科院分区：
文献类型：
--
作者：
Algarra, Manuel;Fernandes, Ana;Casado, Juan
本文研究了两种不同黑胡萝卜（Daucus carota L. ssp）的花青素谱。来自西班牙马拉加Cuevas Bajas （Malaga, Spain）的sativus var. atrorubens Alef.)与Antonina和Purple Haze品种相关的品种及其抗氧化特性。LC-MS检测到的主要花青素分别为花青素3-木糖糖半乳糖苷、花青素3-木糖糖半乳糖苷和花青素3-木糖糖半乳糖苷的辛酸、阿魏酸和香豆酸衍生物。黑胡萝卜中的花青素基本上是酰化的，其含量分别相当于紫霾和安东尼品种总酚含量的25%和50%。以Trolox当量单位测定了两种黑胡萝卜提取物（86.4 +/- 8.0和182.0 +/- 27 μ M TE/100 g fw）的还原能力和自由基清除能力（17.6 +/- 9.0和240.0 +/- 54.0 μ M TE/100 g fw）。黑胡萝卜提取物的抗氧化作用明显高于橙胡萝卜提取物。总之，本研究强调了Cuevas Bajas黑胡萝卜富含花青素，具有显著的抗氧化能力和良好的营养价值。(C) 2013爱思唯尔公司版权所有。
The present work deals with the study of the anthocyanin profile of two different black carrots (Daucus carota L. ssp. sativus var. atrorubens Alef.) cultivars, associated with Antonina and Purple Haze varieties, from Cuevas Bajas (Malaga, Spain) and some of their antioxidant features. The main anthocyanins detected by LC-MS were found to correspond to five cyanidin-based anthocyanins: cyanidin 3-xylosylglucosylgalactoside, cyanidin 3-xylosylgalactoside and the sinapic, ferulic and coumaric acids derivative of cyanidin 3-xylosylglucosylgalactoside. The anthocyanins present in the black carrots were essentially acylated and their levels were found to correspond to 25% and 50% of the total phenolic content for the Purple Haze and Antonina varieties, respectively. Moreover, the reducing capacity of the two black carrots extracts (86.4 +/- 8.0 and 182.0 +/- 27 mu M TE/100 g fw) and the radical scavenging ability (17.6 +/- 9.0 and 240.0 +/- 54.0 mu M TE/100 g fw) expressed in Trolox equivalents units were determined. The antioxidant features of the black carrot extracts were shown to be significantly higher than those of orange carrots used herein for comparison. Overall, this work highlights the Cuevas Bajas black carrots as rich sources of anthocyanins with significant antioxidant capacities and good nutritional value. (C) 2013 Elsevier Inc. All rights reserved.