The expression of differentiation markers in aquaporin-3 deficient epidermis.
The expression of differentiation markers in aquaporin-3 deficient epidermis.
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DOI：
10.1007/s00403-009-0927-9
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发表时间：
2009-03
影响因子：
3
通讯作者：
Miyachi Y
中科院分区：
文献类型：
--
作者：
Hara-Chikuma M;Takahashi K;Chikuma S;Verkman AS;Miyachi Y
水通道蛋白-3(Aquaporin-3，AQP3)是一种在角质形成细胞质膜上强表达的水/甘油转运蛋白。有证据表明，AQP3促进的水和甘油运输分别参与了角质形成细胞的迁移和增殖。在这里，我们研究了AQP3在角质形成细胞分化中的作用。研究使用了AQP3基因敲除小鼠、原代培养的小鼠角质形成细胞(AQP3基因敲除)、新生人类角质形成细胞(AQP3基因敲除)和人皮肤。细胞用高钙或1-α，25-二羟基维生素D3诱导分化。在对照组和AQP3基因敲除或敲除的角质形成细胞中，分化标志蛋白的表达和分化反应相似。外用全反式维甲酸(RA)，一种已知的角质形成细胞分化和增殖调节剂，在野生型和AQP3缺失的表皮中诱导类似的分化标记蛋白的表达，尽管在AQP3缺失的小鼠中RA诱导的增殖受到抑制。人和小鼠表皮的免疫染色显示，AQP3在增殖细胞中的表达高于分化细胞。我们的结果显示，AQP3对角质形成细胞分化的影响很小，并为AQP3促进细胞增殖的说法提供了进一步的支持。
Aquaporin-3 (AQP3) is a water/glycerol transporting protein expressed strongly at the plasma membrane of keratinocytes. There is evidence for involvement of AQP3-facilitated water and glycerol transport in keratinocyte migration and proliferation, respectively. Here, we investigated the involvement of AQP3 in keratinocyte differentiation. Studies were done using AQP3 knockout mice, primary cultures of mouse keratinocytes (AQP3 knockout), neonatal human keratinocytes (AQP3 knockdown), and human skin. Cells were cultured with high Ca2+ or 1α,25-dihydroxyvitamin D3 (VD3) to induce differentiation. The expression of differentiation marker proteins and differentiating responses were comparable in control and AQP3-knockout or knockdown keratinocytes. Topical application of all-trans retinoic acid (RA), a known regulator of keratinocyte differentiation and proliferation, induced comparable expression of differentiation marker proteins in wildtype and AQP3 null epidermis, though with impaired RA-induced proliferation in AQP3 null mice. Immunostaining of human and mouse epidermis showed greater AQP3 expression in cells undergoing proliferation than differentiation. Our results showed little influence of AQP3 on keratinocyte differentiation, and provide further support for the proposed involvement of AQP3-facilitated cell proliferation.
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影响因子：
6.5
作者：
Rittie, Laure;Varani, James;Fisher, Gary J.
通讯作者：
Fisher, Gary J.
影响因子：
4.6
作者：
Takahashi, H;Ibe, M;Iizuka, H
通讯作者：
Iizuka, H
影响因子：
4.8
作者：
Hara, M;Ma, TH;Verkman, AS
通讯作者：
Verkman, AS
影响因子：
6.5
作者：
Zheng, XJ;Bollag, WB
通讯作者：
Bollag, WB
DOI：
10.1073/pnas.080499597
发表时间：
2000-04-11
影响因子：
11.1
作者：
Ma, TH;Song, YL;Verkman, AS
通讯作者：
Verkman, AS