Arabidopsis thaliana vegetative storage protein (VSP) genes:: gene organization and tissue-specific expression
Arabidopsis thaliana vegetative storage protein (VSP) genes:: gene organization and tissue-specific expression
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DOI：
10.1023/a:1006072014605
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发表时间：
1998-11-01
影响因子：
5.1
通讯作者：
Motoyoshi, F
中科院分区：
文献类型：
--
作者：
Utsugi, S;Sakamoto, W;Motoyoshi, F
我们先前已经在拟南芥中鉴定了两个编码营养贮藏蛋白（VSPs）的cDNA。与大豆不同，大豆叶片中VSPs的积累量很高。拟南芥VSP mRNA在花中丰富。为了了解VSPs在生殖器官发育中的组织特异性表达和可能的作用，本研究对VSPs的相关基因（Vsp 1和Vsp 2）及其推定的启动子进行了表征。基因组序列分析表明，Vsp 1和Vsp 2彼此相似，除了在它们的内含子，这两个基因被组织在一个串联阵列的间隔为6 kb的区域。利用转基因A.本发明涉及一种拟南芥植物，其携带来自Vsp 1或Vsp 2的与细菌B-葡萄糖醛酸酶（GUS）报告基因融合的启动子。Vsp 1启动子在雌蕊中表达，特别是在花柱、子房基部和末端以及角果中表达，而Vsp 2启动子在营养枝、叶柄、花梗和花托中表达。这些结果表明，Vsp 1和Vsp 2的表达可能是在发育过程中调节的。thaliana.在转基因植株中，叶片中脉周围区域的损伤诱导了GUS活性。因此，Vsp 1和Vsp 2启动子似乎具有组织特异性和创伤所需的元件。
We have previously identified two cDNAs encoding vegetative storage proteins (VSPs) in Arabidopsis thaliana. Unlike soybean in which VSPs accumulate at high levels in leaves, A. thaliana VSP mRNAs are abundant in flowers. To understand tissue-specific expression and possible roles of VSPs on reproductive organ development, genes corresponding to VSPs (Vsp1 and Vsp2) and their putative promoters were characterized in this study. Genomic sequence analysis revealed that Vsp1 and Vsp2 resemble each other except in their introns, and that these two genes were organized in a tandem array with an interval of 6 kb in a region. The expression patterns of Vsp1 and Vsp2 were examined using transgenic A. thaliana plants carrying a promoter from Vsp1 or Vsp2 fused to a bacterial B-glucuronidase (GUS) reporter gene. The promoter from Vsp1 expressed its effect in gynoecia, especially in styles, the basal and distal ends of ovaries and in siliques, whereas the promoter from Vsp2 showed its activity in vegetative shoots, petioles, peduncles and receptacles of floral organs. These results suggest that expression of Vsp1 and Vsp2 may be developmentally regulated in A. thaliana. In the transgenic plants, the GUS activity was induced by wounding in an area around the mid-rib of leaves. Therefore, Vsp1 and Vsp2 promoters appear to have elements required for both tissue specificity and wounding.