Map-based cloning of the pear gene MYB114 identifies an interaction with other transcription factors to coordinately regulate fruit anthocyanin biosynthesis
Map-based cloning of the pear gene MYB114 identifies an interaction with other transcription factors to coordinately regulate fruit anthocyanin biosynthesis
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DOI：
10.1111/tpj.13666
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发表时间：
2017-11-01
期刊：
影响因子：
7.2
通讯作者：
Wu, Jun
中科院分区：
文献类型：
--
作者：
Yao, Gaifang;Ming, Meiling;Wu, Jun
红色水果因其丰富的花色苷和外观而受到消费者的欢迎和广泛接受。研究了红皮梨花色素苷合成调控的分子机制，并对花色素苷合成的基因和转录因子进行了鉴定。本研究通过对中国梨（Pyrus bretschneideri）群体中位于第5连锁群上的红皮色数量性状基因座（QTL）的连锁分析，发现了一个R2 R3 MYB TF候选基因PyMYB 114。通过瞬时转化烟草（Nicotinana tabacum）叶片、草莓（Fragaria）和梨果实，验证了PyMYB 114的功能，导致花青素的生物合成。抑制PyMYB 114可以抑制红皮梨花色素苷的生物合成。通过双荧光素酶报告系统和酵母双杂交试验，发现ERF/AP 2 TF PyERF 3与PyMYB 114及其伴侣PybHLH 3相互作用，共同调节花青素苷的生物合成。此外，PyMYB 114和PyMYB 10的转录本丰度具有相关性，将这两个基因共转化烟草和草莓后，花青素苷的合成得到了增强。这种相互作用网络为研究果实着色和不同转铁蛋白之间的相互作用以调节花青素的生物合成提供了新的视角。意义陈述红色果实因其外观和花青素含量丰富而受到广泛欢迎。然而，红皮梨着色的分子机制在不同的遗传背景之间是不一致的。通过QTL定位鉴定了PyMYB 114，揭示了其与PyERF 3和PybHLH 3互作协同调控梨花色素苷合成的功能。在烟草和草莓中证实了PyMYB 114和PyMYB 10的相互作用导致花青素苷生物合成的增强。
Red fruits are popular and widely accepted by consumers because of an enhanced appearance and enriched anthocyanins. The molecular mechanism of anthocyanin regulation in red-skinned pear (Pyrus) has been studied, and the genes encoding the biosynthetic steps and several transcription factors (TFs) have been characterized. In this study, a candidate R2R3 MYB TF, PyMYB114, was identified by linkage to the quantitative trait loci (QTL) for red skin color on linkage group 5 in a population of Chinese pear (Pyrus bretschneideri). The function of PyMYB114 was verified by transient transformation in tobacco (Nicotinana tabacum) leaves and strawberry (Fragaria) and pear fruits, resulting in the biosynthesis of anthocyanin. Suppression of PyMYB114 could inhibit anthocyanin biosynthesis in red-skinned pears. The ERF/AP2 TF PyERF3 was found to interact with PyMYB114 and its partner PybHLH3 to co-regulate anthocyanin biosynthesis, as shown by a dual luciferase reporter system and a yeast two-hybrid assay. In addition, the transcript abundance of PyMYB114 and PyMYB10 were correlated, and co-transformation of these two genes into tobacco and strawberry led to enhanced anthocyanin biosynthesis. This interaction network provides insight into the coloration of fruits and the interaction of different TFs to regulate anthocyanin biosynthesis.Significance Statement Red-colored fruits are popular and widely accepted because of an enhanced appearance and enriched anthocyanins. However, the molecular mechanisms underlying the coloration of red-skinned pears are inconsistent between different genetic backgrounds. PyMYB114 was identified by QTL mapping, and its function in interacting with PyERF3 and PybHLH3 to co-regulate anthocyanin biosynthesis of pear was revealed. An interaction of PyMYB114 and PyMYB10 leading to enhanced anthocyanin biosynthesis was verified in tobacco and strawberry.