Stages in the life-cycle of Leishmania donovani
Stages in the life-cycle of Leishmania donovani
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DOI：
10.1016/0035-9203(60)90057-2
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发表时间：
1960-01-01
期刊：
影响因子：
--
通讯作者：
HOYLE, G. F.
中科院分区：
文献类型：
--
作者：
PULVERTAFT, R. J. V.;HOYLE, G. F.
对杜氏利什曼原虫鞭毛虫感染细胞的第一阶段进行了观察，组织来自仓鼠和鼠源，培养在马和兔血清加一些酵母膏和汉克液的混合液中。只有单核细胞很容易进入。寄生虫穿透细胞，鞭毛向后延伸。一旦进入细胞，利什曼原虫就可以在鞭毛的引导下自由活动。单核细胞和包含的寄生虫都会迅速分解，但寄生虫通常会分解，并在吞噬细胞分解之前被部分消化。当它们死亡时，利什曼原虫的小体变成空泡化，鞭毛末端出现一个小水滴。|摘要作者：A.C.沃尔顿
Observation of the first stages of infection of cells by the flagellates of Leishmania donovani was carried out using tissues from both hamster and mouse origin raised in a culture medium of a mixture of horse and rabbit sera plus some yeast extract and Hank's fluid. Only monocytes were readily entered. The parasite penetrated the cell with the flagellum trailing. Once inside the cells, the Leishmania then moved about freely with the flagellum in the lead. Both the monocyte and the contained parasites break down quickly, but the parasites usually break down and are partially digested before the phagocyte breaks up. As they die, the Leishmania bodies become vacuolated and a small drop-let appears at the end of the flagellum. || ABSTRACT AUTHORS: A. C. Walton