Comparison of physiological functions between neuromedin U-related peptide and neuromedin S-related peptide in the rat central nervous system
Comparison of physiological functions between neuromedin U-related peptide and neuromedin S-related peptide in the rat central nervous system
复制标题
神经调节肽U相关肽和神经调节肽S相关肽在大鼠中枢神经系统中的生理功能比较
DOI：
10.1016/j.bbrc.2020.11.025
复制
发表时间：
2021
影响因子：
3.1
通讯作者：
Nakahara Keiko
中科院分区：
文献类型：
--
作者：
Tanaka Yukie;Taguchi Shimon;Maruyama Keisuke;Mori Kenji;Miyazato Mikiya;Kangawa Kenji;Murakami Noboru;Nakahara Keiko
神经介肽U（NMU）和神经介肽S（NMS）的前体分别产生两种新的肽，神经介肽U受体相关肽（NREP）和神经介肽S受体相关肽（NSRP），因为这些前体具有用于蛋白水解加工的多个共有序列。我们的研究小组以前已经证明，这两种新肽之一，BMPs，刺激体温和运动活动，但不是食物摄入。然而，另一种肽NSRP的生理功能仍不清楚。因此，本研究的目的是表征NMU/NMS肽家族靶向的大鼠大脑区域的差异，包括NSEP和NSRP，以及它们的生理功能。首先，我们探索了c-Fos表达的区域，脑室内（i. c. v.）注射NMU和NMS后，发现这些比下丘脑中i. c. v.注射NMU和NMS后少，可能是因为NMU和NSRP不能激活NMU/NMS受体。在腹侧下托，这是海马的一个区域，c-Fos的表达是明显的，只有在i. c. v.注射后的CIPs。我们还研究了NSRP对摄食量、体温和自发活动的影响。与BVP一样，NSRP增加体温和运动活性，但不增加食物摄入，表明NSRP也是一种功能性肽。然而，NSRP的这些作用明显弱于CNORP。这些发现表明，在特异性受体，或在其各自的生物活性的亲和力的差异，BMPs和/或NSRP。
Two novel peptides, neuromedin U precursor-related peptide (NURP) and neuromedin S precursor-related peptide (NSRP), are produced from neuromedin U (NMU) and neuromedin S (NMS) precursors, respectively, as these precursors have multiple consensus sequences for proteolytic processing. Our group has shown previously that one of these two novel peptides, NURP, stimulates body temperature and locomotor activity, but not food intake. However, the physiological function of the other peptide, NSRP, has remained unclear. Therefore, the aim of this study was to characterize differences in the regions of the rat brain targeted by the NMU/NMS peptide family, including NURP and NSRP, and their physiological functions. First, we explored the regions of c-Fos expression after intracerebroventricular (i.c.v.) injection of NURP and NSRP and found that these were fewer than after i.c.v. injection of NMU and NMS in the hypothalamus, possibly because NURP and NSRP cannot activate NMU/NMS receptors. In the ventral subiculum, which is one region of the hippocampus, c-Fos expression was evident only after i.c.v. injection of NURP. We also examined the effects of NSRP on food intake, body temperature and locomotor activity. Like NURP, NSRP increased both body temperature and locomotor activity, but not food intake, indicating that NSRP is also a functional peptide. However, these effects of NSRP were distinctly weaker than those of NURP. These findings suggest differences in the affinity of NURP and/or NSRP for specific receptors, or in their respective biological activities.