Distribution of eosinophil granulocytes and mast cells in the reproductive tract of female goats in the preimplantation phase
Distribution of eosinophil granulocytes and mast cells in the reproductive tract of female goats in the preimplantation phase
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DOI：
10.1007/s11259-009-9203-x
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发表时间：
2009-08-01
影响因子：
2.2
通讯作者：
Uslu, Baris Atalay
中科院分区：
文献类型：
--
作者：
Karaca, Turan;Yoruk, Mecit;Uslu, Baris Atalay
授精后嗜酸性粒细胞和肥大细胞的变化可能会影响概念植入，但有关哺乳动物生殖道中此类细胞数量的信息有限。本研究调查了雌性山羊生殖道器官中嗜酸性粒细胞和肥大细胞（MC）的植入前分布。子宫、子宫颈和输卵管样品在屠宰时获取。在磷酸盐缓冲溶液中清洗子宫角（每只动物至少含有一个胚胎）。将组织在 10% 中性缓冲福尔马林、Carnoy 溶液和 Mota 固定剂（碱性醋酸铅）中固定 48 小时，然后包埋在石蜡中。六微米厚的切片用刚果红（对于嗜酸性粒细胞）和甲苯胺蓝在 1% 水溶液中在 pH 1.0 下染色 5 分钟（对于 MC）。在子宫中，MC 在子宫肌层中的数量最多。与对照组相比，植入前（实验）组（通过 7 天阴道内海绵与 0.3 g P-4 同步周期）的子宫、子宫和输卵管中观察到较高的 MC 数量（P < 0.05）。实验组嗜酸性粒细胞数显着高于对照组（P < 0.05）。这些结果表明山羊生殖道器官中嗜酸性粒细胞和 MC 数量与植入前相关的变化。
Changes in eosinophil granulocytes and mast cells post-insemination may affect conceptus implantation, but information regarding the numbers of such cells in the mammalian reproductive tract is limited. This study investigated the preimplantation distribution of eosinophil granulocytes and mast cells (MCs) in the reproductive tract organs of female goats. Uterus, uterine cervix and uterine tubes samples were obtained at slaughter. Cornu uteri were washed in phosphate buffer solution (each animal contained at least one embryo). Tissues were fixed in 10% neutral buffered formol, Carnoy solution and Mota's fixative (basic lead acetate) for 48 h and embedded in paraffin. Six-micrometre-thick sections were stained with Congo red (for eosinophil granulocytes) and toluidine blue in 1% aqueous solution at pH 1.0 for 5 min (for MCs). In the uterus, MCs occurred in highest numbers in the myometrium. Higher MC numbers were observed in uterus, uterine and uterine tubes in the preimplantation (experimental) group (cycle synchronised through 7 days intravaginal sponge with 0.3 g P-4) compared with the control group (P < 0.05). Eosinophil granulocyte numbers were significantly higher in the experimental group than in the control group (P < 0.05). These results indicate preimplantation-related changes in numbers of eosinophil granulocytes and MCs in goat reproductive tract organs.