IDENTIFICATION OF AN EXTRACELLULAR PROTEIN OF LISTERIA-MONOCYTOGENES POSSIBLY INVOLVED IN INTRACELLULAR UPTAKE BY MAMMALIAN-CELLS
IDENTIFICATION OF AN EXTRACELLULAR PROTEIN OF LISTERIA-MONOCYTOGENES POSSIBLY INVOLVED IN INTRACELLULAR UPTAKE BY MAMMALIAN-CELLS
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DOI：
10.1128/iai.57.1.55-61.1989
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发表时间：
1989-01-01
影响因子：
3.1
通讯作者：
GOEBEL, W
中科院分区：
文献类型：
--
作者：
KUHN, M;GOEBEL, W
最近分离出单核细胞增生李斯特菌的突变体，其在主要胞外蛋白（p60）的合成中受损。如本研究所示，p60突变体已丧失侵入非专职吞噬3 T6小鼠成纤维细胞的能力。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳这些突变体的上清液蛋白表明，没有其他细胞外蛋白被改变，在这些突变体。p60突变体形成长的细胞链，其在用部分纯化的p60处理后分解成正常大小的单个细菌。这些分解的细菌细胞能够侵入3 T6细胞。通过超声处理物理破坏细胞链产生类似的单细胞，然而，这是非侵入性的。用野生型p60处理这些超声处理的突变细胞恢复了它们侵袭3 T6细胞的能力。
Mutants of Listeria monocytogenes were recently isolated which are impaired in the synthesis of a major extracellular protein (p60). As shown in this investigation, the p60 mutants have lost the capability of invading nonprofessional phagocytic 3T6 mouse fibroblast cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of supernatant proteins of these mutants indicated that no other extracellular protein was altered in these mutants. The p60 mutants formed long cell chains which disaggregated to normal-sized single bacteria upon treatment with partially purified p60. These disaggregated bacterial cells were able to invade 3T6 cells. Physical disruption of the cell chains by ultrasonication produced similar single cells which were, however, noninvasive. Treatment of these ultrasonicated mutant cells with wild-type p60 restored their ability to invade 3T6 cells.