Isolation and primary structure of a peptide from the corpora cardiaca of Heliothis zea with adipokinetic activity.
Isolation and primary structure of a peptide from the corpora cardiaca of Heliothis zea with adipokinetic activity.
复制标题
具有脂肪运动活性的玉米夜蛾贲门肽的分离和一级结构。
DOI：
10.1016/0006-291x(86)90038-0
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发表时间：
1986
影响因子：
3.1
通讯作者：
Dora K. Hayes
中科院分区：
文献类型：
--
作者：
Howard Jaffe;Ashok K. Raina;Clark T. Riley;Blair A. Fraser;G.Mark Holman;Renée M. Wagner;Richard L. Ridgway;Dora K. Hayes
从玉米夜蛾Heliothis zea的贲门体中分离出脂肪运动激素，并通过反相高效液相色谱法纯化。一级结构 pGlu-Leu-Thr-Phe-Thr-Ser-Ser-Trp-Gly-NH2 通过用焦谷氨酸氨基肽酶解封闭的肽的自动气相 Edman 降解和快原子轰击质谱法测定。该激素是合成的，天然和合成材料具有相同的色谱、光谱和生物学特性。发现该肽在 H 中具有脂质动员活性。玉米成人。
An adipokinetic hormone was isolated from the corpora cardiaca of the corn ear worm moth,Heliothis zea, and purified by reversed phase high performance liquid chromatography. The primary structure, pGlu-Leu-Thr-Phe-Thr-Ser-Ser-Trp-Gly-NH2, was determined by automated gas-phase Edman degradation of the peptide deblocked with pyroglutamic aminopeptidase, and by fast atom bombardment mass spectrometry. The hormone was synthesized and the natural and synthetic material had identical chromatographic, spectroscopic, and biological properties. The peptide was found to have lipid mobilizing activity inH. zeaadults.