Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals
Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals
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DOI：
10.1111/1365-2664.12467
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发表时间：
2015-08-01
影响因子：
5.7
通讯作者：
Maddison, Ben C.
中科院分区：
文献类型：
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作者：
Rees, Helen C.;Gough, Kevin C.;Maddison, Ben C.
在Rees埃塔尔.（2014年b），我们审查了环境DNA（eDNA）监测水生种群的现状。我们的目的是集中讨论所使用的方法，eDNA分析作为生态学调查工具的应用，并包括一些创新的想法，使用eDNA在保护和管理。埃塞尔埃塔尔（2015）声称里斯埃塔尔的分析。（2014 b）和其他出版物强调了该方法的缺点，他们建议应该淡化一些结论。他们的许多论点都涵盖在我们的原始文件（里斯埃塔尔，2014 b）;然而，他们指出应鼓励采用建模方法，我们完全同意这一建议。埃塞尔埃塔尔（2015）还声称，我们忽视了有两个来源的不完善检测（在现场水平和实验室水平）。我们认为，我们的审查文件意味着这一点。合成和应用。埃塞尔埃塔尔（2015）重申了原始论文中提出的许多观点，但确实涵盖了一些额外的领域，这些领域改善了关于环境DNA（eDNA）使用的辩论。这两个评论（Mrsel埃塔尔，2015年）和我们的反驳清楚地强调，详细的实验室协议和严格的现场采样设计是关键因素，需要在文献中进行充分的报告，以便进行实验比较和复制。任何eDNA检测新方法的开发都应该直接与已建立的用于检测被调查物种或栖息地的金标准方法进行比较。没有一个问题是在Eschelsel埃塔尔。（2015）将改变我们的主要结论。（2015）重申了原始论文中提出的许多观点，但确实涵盖了一些额外的领域，这些领域改善了关于环境DNA（eDNA）使用的辩论。这两个评论（Mrsel埃塔尔，2015年）和我们的反驳清楚地强调，详细的实验室协议和严格的现场采样设计是关键因素，需要在文献中进行充分的报告，以便进行实验比较和复制。任何eDNA检测新方法的开发都应该直接与已建立的用于检测被调查物种或栖息地的金标准方法进行比较。没有一个问题是在Eschelsel埃塔尔。（2015）将改变我们的主要结论。
In Rees etal. (2014b), we reviewed the current status of environmental DNA (eDNA) to monitor aquatic populations. Our aim was to focus on discussion of methodologies used, application of eDNA analysis as a survey tool in ecology, and to include some innovative ideas for using eDNA in conservation and management. Roussel etal. (2015) claim that analysis of Rees etal. (2014b) and other publications highlights the downsides of the method, and they suggest that some conclusions should be toned down. Many of their arguments were covered in our original paper (Rees etal., 2014b); however, they make the point that modelling approaches should be encouraged, and we fully agree with this suggestion. Roussel etal. (2015) also claim that we neglected to recognize that there are two sources of imperfect detection (at the field level and at the laboratory level). We feel that our review paper implies this point.Synthesis and applications. Roussel etal. (2015) reiterate many of the points made in the original paper but do cover some additional areas that improve the debate on the use of environmental DNA (eDNA). Both the comment (Roussel etal., 2015) and our rebuttal clearly highlight that detailed laboratory protocols and rigorous field sampling design are crucial factors which require sufficient reporting in the literature to allow for experimental comparison and replication. Any development of a new method for eDNA detection should be compared directly with established gold standard' methods for the detection of the species or habitat under investigation. None of the issues raised in Roussel etal. (2015) would alter our main conclusions.Roussel etal. (2015) reiterate many of the points made in the original paper but do cover some additional areas that improve the debate on the use of environmental DNA (eDNA). Both the comment (Roussel etal., 2015) and our rebuttal clearly highlight that detailed laboratory protocols and rigorous field sampling design are crucial factors which require sufficient reporting in the literature to allow for experimental comparison and replication. Any development of a new method for eDNA detection should be compared directly with established gold standard' methods for the detection of the species or habitat under investigation. None of the issues raised in Roussel etal. (2015) would alter our main conclusions.