X-linked female-sterile loci in Drosophila melanogaster.
X-linked female-sterile loci in Drosophila melanogaster.
复制标题
果蝇中 X 连锁雌性不育位点。
DOI：
10.1093/genetics/113.3.695
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发表时间：
1986
期刊：
影响因子：
3.3
通讯作者：
Mahowald,AP
中科院分区：
文献类型：
--
作者：
Perrimon,N;Mohler,D;Engstrom,L;Mahowald,AP
我们研究了仅在卵子形成过程中特异性需要的x连锁位点的数量。在Gans和Mohler的两个突变筛选中获得的女性不育（fs）突变的互补分析表明，Gans收集的两个或多个突变等位基因所代表的任何fs位点通常都存在于Mohler的收集中。然而，当一个位点在一个集合中由单个等位基因代表时，它通常不存在于另一个集合中。我们提出，这种差异是由于大多数由少于两个突变等位基因代表的“fs位点”实际上是重要（合子致死）基因，而fs等位基因是这些基因的次胚突变。为了支持这一假设，我们已经在这些“fs基因座”中确定了12个致命等位基因。目前的分析可能已经确定了通过x染色体突变检测到的所有母体效应致死位点，并使我们能够重新评估果蝇基因组中“卵巢特异性fs”位点的数量。最后，对大量的fs突变进行生殖系克隆分析，以估计生殖系和体细胞衍生物对卵发生和胚胎发育的相对贡献。所有测试的母体效应致死基因座都依赖于生殖系。
We have examined the number ofX-linked loci specifically required only during oogenesis. Complementation analyses among female-sterile (fs) mutations obtained in two mutagenesis screens—Gans' and Mohler's—indicate that any fs locus represented by two or more mutant alleles in Gans' collection are usually present in Mohler's collection. However, when a locus is represented by a single allele in one collection, it is generally not present in the other collection. We propose that this discrepancy is due to the fact that most "fs loci" represented by less than two mutant alleles are, in fact, vital (zygotic lethal) genes, and that the fs alleles are hypomorphic mutations of such genes. In support of this hypothesis we have identified lethal alleles at 12 of these "fs loci." The present analysis has possibly identified all maternal-effect lethal loci detectable by mutations on theXchromosome and has allowed us to reevaluate the number of "ovary-specific fs" loci in the Drosophila genome. Finally, germline clone analysis of a large number of fs mutations was performed in order to estimate the relative contribution of germline and somatic cell derivatives to oogenesis and to embryonic development. All the maternal-effect lethal loci tested are germline-dependent.