Expression level of methanol-inducible peroxisomal proteins and peroxisome morphology are affected by oxygen conditions and mitochondrial respiratory pathway function in the methylotrophic yeastCandida boidinii.
Expression level of methanol-inducible peroxisomal proteins and peroxisome morphology are affected by oxygen conditions and mitochondrial respiratory pathway function in the methylotrophic yeastCandida boidinii.
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甲基营养酵母博伊丁假丝酵母中甲醇诱导的过氧化物酶体蛋白的表达水平和过氧化物酶体形态受到氧条件和线粒体呼吸途径功能的影响。
DOI：
10.1111/1567-1364.12040
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发表时间：
2013
期刊：
影响因子：
3.2
通讯作者：
and T. Nakagawa
中科院分区：
文献类型：
--
作者：
S. Fujimura;H. Yurimoto;S. Kurimoto;Y.Matsufuji;T. Ito;T. Hayakawa;N. Tomizuka;Y. Sakai;and T. Nakagawa
在甲基营养型酵母中，博伊丁假丝酵母，甲醇诱导的过氧化物酶体蛋白，例如醇氧化酶（AOD），二羟丙酮合酶（DAS），过氧化物酶体谷胱甘肽过氧化物酶（Pmp20），仅在有氧条件下诱导，而表达的PMP47编码的过氧化物酶体膜蛋白Pmp47是独立的氧条件。甲醇诱导的过氧化物酶体酶的表达被抑制的线粒体呼吸链的抑制。在p0突变株中，尽管有氧存在，但它们的诱导水平很低，而PMP 47的表达不受影响。综上所述，这些事实表明C. Boidiniican感知氧条件，线粒体呼吸功能可能对过氧化物酶体蛋白的甲醇诱导基因表达的诱导具有深远的影响。过氧化物酶体形态也受到氧条件和呼吸功能的影响。在缺氧条件下或呼吸抑制条件下，甲醇诱导的细胞中含有小的过氧化物酶体，表明过氧化物酶体的生物合成和蛋白质进口机械不受氧条件的影响，但过氧化物酶体形态依赖于过氧化物酶体基质蛋白的诱导。
In the methylotrophic yeast,Candida boidinii, methanol-inducible peroxisomal proteins, for example alcohol oxidase (AOD), dihydroxyacetone synthase (DAS), and peroxisomal glutathione peroxidase (Pmp20), were induced only under aerobic conditions, while expression ofPMP47encoding peroxisomal integral membrane protein Pmp47 was independent of oxygen conditions. Expression of the methanol-inducible peroxisomal enzymes was repressed by inhibition of the mitochondrial respiratory chain. In the respiratory-deficient (ρ0) mutant strain, their induction was at very low levels despite the presence of oxygen, whereas the expression ofPMP47was unaffected. Taken together, these facts indicate thatC. boidiniican sense oxygen conditions, and that mitochondrial respiratory function may have a profound effect on induction of methanol-inducible gene expression of peroxisomal proteins. Peroxisome morphology was also affected by oxygen conditions and respiratory function. Under hypoxic conditions or respiration-inhibited conditions, cells induced by methanol contained small peroxisomes, indicating that peroxisome biogenesis and the protein import machinery were not affected by oxygen conditions but that peroxisome morphology was dependent on induction of peroxisomal matrix proteins.