Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat
Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat
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DOI：
10.1007/s00122-012-1802-1
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发表时间：
2012-05-01
影响因子：
5.4
通讯作者：
Lagudah, Evans S.
中科院分区：
文献类型：
--
作者：
Herrera-Foessel, Sybil A.;Singh, Ravi P.;Lagudah, Evans S.
普通小麦品种 Parula 对所有三种小麦锈病具有高水平的慢锈病成株抗性 (APR)。之前使用 Avocet-YrA/Parula 重组自交系 (RIL) 群体进行的作图研究表明，Parula 对叶锈菌 (Puccinia triticina) 的 APR 至少受三个独立的慢锈病抗性基因控制：7DS 上的 Lr34、1BL 上的 Lr46 和 7BL 上以前未知的基因。使用田间锈病反应和侧翼标记从上述群体中鉴定出两个 F-6 RIL，Arula1 和 Arula2，它们缺乏 Lr34 和 Lr46，但在 7BL 中携带叶锈病抗性基因，特此命名为 Lr68。 Arula1 和 Arula2 与 Apav（来自 Avocet-YrA/Pavon 76 杂交的高度易感品系）杂交，并开发了 396 个 F-4 衍生的 F-5 RIL 用于定位 Lr68。在墨西哥奥夫雷贡城，对 RIL 的叶锈病抗性进行了两年多的表型分析，混合了小麦叶锈病小种 MBJ/SP 和 MCJ/SP。使用 367 个 RIL 建立了与 7BL 上几个 DNA 标记的密切遗传连锁； Psy1-1 和 gwm146 位于 Lr68 两侧，估计分别为 0.5 和 0.6 cM。通过在温室中用 Lr14b 无毒小麦小麦小种 TCT/QB 评估 RIL，研究了 Lr68 与位于同一区域并存在于 Parula、Arula1 和 Arula2 中的小种特异性幼苗抗性基因 Lr14b 之间的关系。尽管在 RIL 中未鉴定出排斥中的 Lr14b 和 Lr68 纯合重组体，但缺乏 Lr68 但具有 Lr14b 的 γ 辐射诱导缺失种群表明 Lr68 和 Lr14b 是不同的基因座。在多种基因型中与 Lr68 紧密连锁的侧翼 DNA 标记可用于选择 APR 至叶锈病。
The common wheat cultivar Parula possesses a high level of slow rusting, adult plant resistance (APR) to all three rust diseases of wheat. Previous mapping studies using an Avocet-YrA/Parula recombinant inbred line (RIL) population showed that APR to leaf rust (Puccinia triticina) in Parula is governed by at least three independent slow rusting resistance genes: Lr34 on 7DS, Lr46 on 1BL, and a previously unknown gene on 7BL. The use of field rust reaction and flanking markers identified two F-6 RILs, Arula1 and Arula2, from the above population that lacked Lr34 and Lr46 but carried the leaf rust resistance gene in 7BL, hereby designated Lr68. Arula1 and Arula2 were crossed with Apav, a highly susceptible line from the cross Avocet-YrA/Pavon 76, and 396 F-4-derived F-5 RILs were developed for mapping Lr68. The RILs were phenotyped for leaf rust resistance for over 2 years in Ciudad Obregon, Mexico, with a mixture of P. triticina races MBJ/SP and MCJ/SP. Close genetic linkages with several DNA markers on 7BL were established using 367 RILs; Psy1-1 and gwm146 flanked Lr68 and were estimated at 0.5 and 0.6 cM, respectively. The relationship between Lr68 and the race-specific seedling resistance gene Lr14b, located in the same region and present in Parula, Arula1 and Arula2, was investigated by evaluating the RILs with Lr14b-avirulent P. triticina race TCT/QB in the greenhouse. Although Lr14b and Lr68 homozygous recombinants in repulsion were not identified in RILs, gamma-irradiation-induced deletion stocks that lacked Lr68 but possessed Lr14b showed that Lr68 and Lr14b are different loci. Flanking DNA markers that are tightly linked to Lr68 in a wide array of genotypes can be utilized for selection of APR to leaf rust.