Effect of Quantum phytase on nutrient digestibility and bone ash in White Leghorn laying hens fed corn-soybean meal-based diets
Effect of Quantum phytase on nutrient digestibility and bone ash in White Leghorn laying hens fed corn-soybean meal-based diets
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DOI：
10.3382/ps.2008-00233
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发表时间：
2009-06-01
期刊：
影响因子：
4.4
通讯作者：
Classen, H. L.
中科院分区：
文献类型：
--
作者：
Hughes, A. L.;Dahiya, J. P.;Classen, H. L.
研究了在玉米-豆粕(CSM)日粮中添加大肠杆菌6-植酸酶(Quantum)对蛋鸡养分消化率和骨灰分的影响。在21~61周龄的白来航鸡(Shaver和Bovan品系)日粮中添加0.35%(阳性对照，PC)、0.25%(阴性对照1，NC1)或0.15%(阴性对照2，NC2)非植酸磷。通过在阴性对照日粮中添加每公斤200、400和600个单位的外源植酸酶，又生产了6个日粮，共9个处理。每个日粮处理x品系亚类重复两次，每次重复6只母鸡。在42周龄时采集粪便和回肠消化样本，以确定营养物质的表观消化率或存留率。分别于42周龄和61周龄取左侧胫骨测定骨灰。未添加的阴性对照组的回肠消化率和粪便蛋白质滞留率显著高于对照组(P<；0.05)。据报道，植酸消化率和回肠蛋白质消化率随着NC1日粮植酸酶水平的增加而线性降低。在NC1处理中添加植酸酶使氨基酸的消化率直线下降，但蛋氨酸和脯氨酸除外。与PC相比，每千克含有400个植酸酶的NC2处理的植酸消化率显著提高。每千克饲粮添加200或400个单位植酸酶的61周龄母鸡胫骨灰分百分比较高(P<；0.05)。与博万母鸡相比，Shaver母鸡的日粮AME和粪便蛋白质滞留率显著高于Bovan母鸡。总体而言，在饲喂缺乏非植酸P的CSM日粮的蛋鸡中，量子植酸酶不能有效地提高营养物质的消化率和存留率。
The efficacy of an Escherichia coli 6-phytase supplementation (Quantum) on nutrient digestibility-retention and bone ash in laying hens fed corn-soybean meal (CSM) diets was investigated. White Leghorn hens (Shaver and Bovan strains) were fed CSM diets containing 0.35% (positive control, PC), 0.25% (negative control 1, NC1), or 0.15% (negative control 2, NC2) nonphytate P from 21 to 61 wk of age. Six more diets were manufactured by supplementing the negative control diets with 200, 400, and 600 units per kilogram of exogenous phytase resulting in a total of 9 treatments. Each dietary treatment x strain subclass was replicated twice with 6 hens per replication. Fecal and ileal digesta samples were collected at 42 wk of age to determine apparent nutrient digestibility or retention. Left tibiae were collected at 42 and 61 wk of age to determine bone ash. The coefficients for ileal digestibility and fecal retention for protein were higher (P < 0.05) for the unsupplemented negative control treatments compared with the PC. A linear reduction in phytate digestibility and ileal protein digestibility was reported with increasing levels of phytase to the NC1 diet. Phytase addition to the NC1 treatment resulted in a linear decrease in the digestibility of amino acids except for methionine and proline. Significantly higher phytate digestibility was demonstrated with the NC2 treatment containing 400 units per kilogram of phytase compared with the PC. Tibial bone ash percentage was higher (P < 0.05) in 61-wk-old hens fed 200 or 400 units per kilogram of phytase-supplemented NC2 diets. Significantly higher diet AME and fecal protein retention were demonstrated for Shaver hens in comparison to the Bovan hens. Overall, the Quantum phytase was not efficacious at improving nutrient digestibility-retention in laying hens fed CSM diets deficient in nonphytate P.