High-performance cation-exchange chromatography and pulsed amperometric detection for the separation, detection, and quantitation of N-alkylated imino sugars in biological samples.
High-performance cation-exchange chromatography and pulsed amperometric detection for the separation, detection, and quantitation of N-alkylated imino sugars in biological samples.
复制标题
高性能阳离子交换色谱和脉冲电流检测，用于生物样品中 N-烷基化亚氨基糖的分离、检测和定量。
DOI：
10.1006/abio.2000.4678
复制
发表时间：
2000
影响因子：
2.9
通讯作者：
T. Butters
中科院分区：
文献类型：
--
作者：
H. Mellor;A. Adam;F. Platt;R. Dwek;T. Butters
The use of imino sugars for the potential treatment of lysosomal glycolipid storage diseases and hepatitis virus infections requires accurate, quantitative measurement of these compounds in biological samples. We demonstrate here the versatility of cation-exchange chromatography and pulsed amperometric detection of a range of compounds that differ in both isometric structure and N-alkyl chain length. Although column retention appears dependent upon residual charge on the imine function, successful isocratic separation can be achieved by secondary hydrophobic interactions. A series of N-alkylated deoxynojirimycin compounds containing C(1-10) alkyl chains are readily separated and detected by pulsed amperometry after cation suppression. Using experimentally derived response factors for imino sugars and measurement of peak areas we have developed a reliable method for quantitatively determining concentrations in solution. A rapid protocol for the removal of protein and contaminants in biological samples is described. This has allowed the successful measurement of imino sugars in animal tissues and will be useful for understanding the factors involved in compound bioavailability and in the design of novel therapeutics.
DOI：
10.1073/pnas.85.10.3289
发表时间：
1988-05-01
影响因子：
11.1
作者：
HARDY, MR;TOWNSEND, RR
通讯作者：
TOWNSEND, RR