Identification of a microsatellite on chromosome 7B showing a strong linkage with yellow pigment in durum wheat (Triticum turgidum L. var. durum)
Identification of a microsatellite on chromosome 7B showing a strong linkage with yellow pigment in durum wheat (Triticum turgidum L. var. durum)
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DOI：
10.1111/j.1601-5223.2001.t01-1-00255.x
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发表时间：
2001-01-01
期刊：
影响因子：
2.7
通讯作者：
Martin, LM
中科院分区：
文献类型：
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作者：
Elouafi, I;Nachit, MM;Martin, LM
本研究旨在鉴定与硬粒小麦黄色素含量相关的QTL。利用124个微卫星、149个扩增片段长度多态性（AFLP）和6个种子贮藏蛋白（SSP）在114个重组自交系（178）群体中构建了硬粒小麦-双子叶植物遗传连锁图谱。该群体已从硬粒小麦栽培品种Omrabi 5和二粒小麦600545之间的杂交获得，并与Omrabi 5回交。该图谱由14条硬粒小麦染色体和一个未知染色体组组成，与已发表的小麦图谱具有较好的同线性。在3个季节中，在3个不同的地点测量了种群中的黄色素。QTL的分析是基于简单和简化的复合区间作图（SIM和sCIM）。在第7染色体组（7AL和7 BL端粒）上检测到3个与黄色素相关的QTL，可解释总变异的62%。在7 BL上，一个主效微卫星（Xgwm 344）的贡献率为53%，而在7AL上，另外两个QTL的贡献率分别为13%和6%。所有QTL均表现出较强的遗传效应和较弱的QTL x E效应。QTL效应在所有环境中是一致的，并且显示出较大的效应。因此，有希望的QTL将用于标记辅助育种，以提高选择效率的黄色素。
The objective of this study is to identify QTLs linked to yellow pigment content in durum wheat. A durum-dicoccoides genetic linkage map was constructed using 124 microsatellites, 149 amplified fragment length polymorphism (AFLPs), and six seed storage proteins (SSP) in a population of 114 recombinant inbred lines (178). The population has been obtained from a cross between a durum cultivar Omrabi5 and Triticum dicoccoides 600545 and backcrossed to Omrabi5. The map consists of 14-durum chromosomes plus an unknown group; and shows a good synteny to the previously published wheat maps. Yellow pigment was measured in the population in three different locations during 3 seasons. Analysis of QTLs was based on simple and simplified composite interval mapping (SIM and sCIM). Three QTLs for yellow pigment were detected on the chromosomal group 7 (7AL and 7BL telomeres) explaining 62% of the total variation. On 7BL, a major microsatellite (Xgwm 344) explained by itself 53%, whereas on 7AL, the other two QTLs have contributed 13 and 6%. All determined QTLs showed a strong genetic effect and a weak QTL x E effect. The QTLs effect was consistent across all environments and showed a large effect. Consequently, promising QTLs will be used in the marker assisted breeding program to enhance the selection efficiency for yellow pigment.