Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide
Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide
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DOI：
10.1039/c6qo00448b
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发表时间：
2017-06-01
影响因子：
5.4
通讯作者：
James, Tony D.
中科院分区：
文献类型：
--
作者：
Sun, Xiaolong;Odyniec, Maria L.;James, Tony D.
基于苯基硼酸（PBA）与茜素红S（ARS）的络合作用，研制了一种检测水溶液中过氧化氢（H2O2）的新型化学传感器。该平台已经证明了其通过比色、荧光和电化学测量检测H2O2的能力。实验观察表明，该系统显示出红移可见的颜色变化，开关荧光响应指示剂（ARS）的释放和打开的电化学信号指示生成苯酚，与H2O2反应后。通过这项工作，我们已经证明，我们的反应为基础的指示剂置换分析（RIA）系统，可以作为一种测定过氧化氢和过氧化氢相关的物种的环境和生理检测。
Based on the complexation of phenylboronic acid (PBA) with Alizarin Red S (ARS), we developed a new chemosensor for the detection of hydrogen peroxide (H2O2) in aqueous media. This platform has demonstrated its ability to detect H2O2 via colorimetric, fluorometric, and electrochemical measurements. The experimental observations reveal that the system displays a red-shifted visible colour change, on-off fluorescence response indicating release of indicator (ARS) and turn-on electrochemical signal indicating generation of phenol, after reaction with H2O2. With this work we have demonstrated that our reaction-based indicator displacement assay (RIA) systems, can be employed as an assay for H2O2 and hydrogen peroxide-related species for environmental and physiological detection.