A novel NK-type homeobox gene, ENK (early embryo specific NK), preferentially expressed in embryonic stem cells
A novel NK-type homeobox gene, ENK (early embryo specific NK), preferentially expressed in embryonic stem cells
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DOI：
10.1016/s1567-133x(03)00005-x
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发表时间：
2003-03-01
影响因子：
1.2
通讯作者：
Li, H
中科院分区：
文献类型：
--
作者：
Wang, SH;Tsai, MS;Li, H
为了确定新的同源盒基因参与早期胚胎发育，我们进行了简并寡核苷酸聚合酶链反应（PCR）筛选小鼠胚胎干细胞（ES）的cDNA文库。ENK（early embryo specific expression NK family）是发现具有早期胚胎阶段特异性表达的几个基因之一。全长ENK cDNA的克隆和其基因组结构的特点。小鼠ENK跨越7.1 kb，编码4个外显子，定位于小鼠染色体6 F2。反转录-PCR和北方印迹分析表明ENK在小鼠胚胎中优先表达，在囊胚中表达量较高。整体原位杂交分析进一步表明，ENK mRNA主要存在于内细胞团的囊胚。ENK在未分化的ES细胞中的表达明显高于视黄酸分化的ES细胞和胚胎体。ENK的表达略有下降，在早期的原始外胚层样（EPL）细胞和缺席后9.5天的胚胎阶段。ENK基因是哺乳动物胚胎发育过程中在ES细胞中优先表达的少数同源框编码基因之一。(C)2003 Elsevier Science B. V.保留所有权利。
In order to identify novel homeobox-containing genes involved in early embryonic development, we conducted a degenerate oligonucleotide polymerase chain reaction (PCR) screening of a murine embryonic stem (ES) cell cDNA library. ENK (early embryo specific expression NK family) was one of several genes isolated that was found to exhibit early embryo stage-specific expression. The full-length ENK cDNA was cloned and its genomic organization was characterized. Murine ENK spans 7.1 kb, encodes four exons and maps to mouse chromosome 6F2. Reverse transcriptase-PCR and Northern blot analyses show that ENK is preferentially expressed in pre-implantation mouse embryos and a higher level in blastocysts. Whole-mount in situ hybridization analysis further demonstrates that ENK mRNA is present predominantly in the inner cell mass of blastocysts. The expression of ENK is markedly higher in undifferentiated ES cells than in retinoic acid differentiated ES cells and embryonic bodies. ENK expression slightly decreased in early primitive ectoderm-like (EPL) cells and was absent after the 9.5-day embryo stages. ENK is one of the few homeobox-encoding genes preferentially expressed in ES cells during mammalian embryogenesis. (C) 2003 Elsevier Science B.V. All rights reserved.