IDENTIFICATION AND DEVELOPMENT OF A GENETICALLY CLOSELY-LINKED CARBOXYLESTERASE FAMILY OF THE MOUSE-LIVER
IDENTIFICATION AND DEVELOPMENT OF A GENETICALLY CLOSELY-LINKED CARBOXYLESTERASE FAMILY OF THE MOUSE-LIVER
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DOI：
10.1016/0305-0491(85)90475-4
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发表时间：
1985-01-01
影响因子：
2.2
通讯作者：
VONDEIMLING, O
中科院分区：
文献类型：
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作者：
BERNING, W;DELOOZE, SM;VONDEIMLING, O
六种羧酸酯酶同工酶（即 ES-1、ES-6、ES-9、ES-20、ES 22 和 ES-24）由家鼠第 8 号染色体上的酯酶基因簇 1 控制，利用其生化、遗传和发育特征在肝脏上清液中进行电泳鉴定。 ES-1和ES-20以肝脏特异性形式表达。 6 种同工酶的围产期和产后发育表明，它们在基因水平上受到单独调节，尽管与遗传无关的酯酶相比，这些同工酶是作为一个整体受到调节的。祖先酯酶结构基因重复基因复制后进化分歧的概念被扩展到涵盖与多基因家族相关的时间（调节）基因的分歧。时间基因的等位基因变异比相应的结构基因的等位基因变异更有限。
Six carboxylesterase isozymes (viz. ES-1, ES-6, ES-9, ES-20, ES 22 and ES-24), governed by esterase gene cluster 1 on chromosome 8 of the house mouse, were identified electrophoretically in liver supernatants using their biochemical, genetic and developmental characteristics. ES-1 and ES-20 were expressed as liver-specific forms. The peri- and postnatal development of the 6 isozymes indicated that they were individually regulated at the genetic level, although the isozymes were regulated as a group when compared to genetically unrelated esterases. The concept of evolutionary divergence following repeated gene duplication of an ancestral esterase structural gene was extended to cover divergence of the temporal (regulatory) genes associated with the multigene family. Allelic variation of the temporal genes was more limited than that of the corresponding structural genes.