Distribution of D-Aspartate-Immunopositive Cells in the Ganglion Cell Layer of Adult Rat Retina
Distribution of D-Aspartate-Immunopositive Cells in the Ganglion Cell Layer of Adult Rat Retina
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DOI：
10.1159/000321944
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发表时间：
2010-11
影响因子：
2.1
通讯作者：
M. Sasoh;Ning Ma;Hisashi Matsubara;K. Matsunaga;Y. Uji
中科院分区：
文献类型：
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作者：
M. Sasoh;Ning Ma;Hisashi Matsubara;K. Matsunaga;Y. Uji
背景/目的：D-天冬氨酸是一种重要的视网膜神经递质或神经调质。本研究的目的是探讨D-天冬氨酸免疫阳性细胞在神经节细胞层的细胞类型和分布。方法：Wister大鼠用5%戊二醛灌流固定。使用D-天冬氨酸和谷氨酸或γ-氨基丁酸抗体对视网膜的石蜡切片进行化学双标记。D-天冬氨酸标记后，在神经节细胞层的血管周围和非血管周围区域的细胞密度分别测量的中央，midperipheral，和周边区，全挂载视网膜。结果：在石蜡切片中，神经节细胞层中的一些谷氨酸免疫反应细胞呈D-天冬氨酸免疫反应。这些D-天冬氨酸阳性细胞在神经节细胞层更频繁地观察到沿着视网膜血管在整个安装的视网膜。D-天冬氨酸阳性细胞密度在血管周围区域显著高于非血管周围区域的中央、中外周和外周区（p < 0.0001）。结论：在神经节细胞层中，D-天冬氨酸免疫阳性细胞被证明是神经节细胞，发现它们沿着大的视网膜血管如小动脉或小静脉分布更密集。
Background/Aims:D-aspartate is an important candidate for retinal neurotransmitter or neuromodulator. The purpose of this study was to investigate the cells type and distribution of the D-aspartate-immunopositive cells in the ganglion cell layer. Methods: Wister rats were fixed by perfusion with 5% glutaraldehyde. Paraffin sections of the retina were immunohistochemically double-labeled using D-aspartate and glutamate or γ-aminobutyric acid antibodies. After labeling the whole-mount retina by D-aspartate, the cell densities of both the perivascular and nonperivascular areas in the ganglion cell layer were measured for the central, midperipheral, and peripheral zones, respectively. Results: Within the paraffin section, some of the glutamate-immunoreactive cells in the ganglion cell layer were D-aspartate immunoreactive. These D-aspartate-positive cells in the ganglion cell layer were more frequently observed along the retinal vessels in the whole-mount retina. The D-aspartate-positive cell densities in the perivascular area were significantly higher than those in the nonperivascular area for the central, midperipheral, and peripheral zones (p < 0.0001). Conclusion: In the ganglion cell layer, the D-aspartate-immunopositive cells proved to be the ganglion cells, with their distribution found to be denser along the large retinal vessels such as the arterioles or venules.