Molecular identification of Wolbachia, the agent of cytoplasmic incompatibility in Drosophila simulans, and variability in relation with host mitochondrial types
Molecular identification of Wolbachia, the agent of cytoplasmic incompatibility in Drosophila simulans, and variability in relation with host mitochondrial types
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沃尔巴克氏体（果蝇细胞质不相容性的病原体）的分子鉴定，以及与宿主线粒体类型相关的变异性
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
M. Solignac
中科院分区：
文献类型：
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作者：
F. Rousset;D. Vautrin;M. Solignac
Wolbachia的16S核糖体DNA片段的序列，立克次体样微生物负责在果蝇的细胞质不相容性，已获得聚合酶链反应（PCR）扩增后。与其他真细菌序列的比较使我们能够将这些内共生体分配给紫色细菌的a α亚群。已获得四个相关序列的微生物所携带的8个同雌株系代表的三种线粒体类型的D。simulans。它们的同源性和分化水平与线粒体DNA的不平行，表明发生了几次独立的感染。细菌的不亲和性与以前鉴定的不亲和性类型之间没有直接关系。
Sequences of a segment of the 16S ribosomal DNA of Wolbachia, a rickettsia-like microorganism responsible for cytoplasmic incompatibility in Drosophila simulans, have been obtained after polymerase chain reaction (PCR) amplification. Their comparison with other eubacterial sequences allows us to assign these endosymbionts to the a α subdivision of purple bacteria. Four related sequences have been obtained for microorganisms carried by eight isofemale lines representative of the three mitochondrial types of D. simulans. Their phylogeny and level of divergence do not parallel that of the mitochondrial DNA, suggesting that several independent infections occurred. There is no direct relation between bacterial phylogeny and formerly identified incompatibility types.