Ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media
Ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media
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DOI：
10.1016/j.actbio.2011.07.016
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发表时间：
2011-12-01
影响因子：
9.7
通讯作者：
Planell, J.
中科院分区：
文献类型：
--
作者：
Gustavsson, J.;Ginebra, M. P.;Planell, J.
溶液介导的表面反应发生在大多数以磷酸钙为基础的生物材料中，并可能影响细胞的反应。要合理地将体外观察到的这些过程外推到体内表现，需要对潜在的机制有深刻的理解。因此，我们通过将缺钙羟基磷灰石(CDHA)暴露在不同化学成分的标准细胞培养液(DMEM和McCoy培养液，含和不含成骨补充剂和血清蛋白)中不同时间，系统地研究了CDHA的离子反应性。主要胞外离子的动力学离子相互作用研究表明，在与CDHA的初始接触过程中(48h)，细胞外主要离子对Ca~(2+)(类似于50%的吸附)和K+(类似于8%)的非线性吸附以及所有介质的酸化。有趣的是，无机磷(P-I)从McCoy培养液(类似于50%)或使用含有β-甘油磷酸的成骨培养液中被吸附，而不是从DMEM培养液中吸附。非线性吸附数据可用拟一级和准二级吸附模型较好地描述。在较长的接触时间(21天)下，随着培养液的频繁更新，在整个实验过程中，Ca~(2+)的吸附保持不变，而P-I在McCoy介质中的吸附逐渐减少。与之形成鲜明对比的是。使用DMEM培养液时，CDHA随着时间的延长开始缓慢释放P-I。红外光谱表明，CDHA在培养介质中具有碳化的表面化学，表明碳酸盐在CDHA的离子反应性中起着关键作用。我们的数据显示，水环境的不同组成可能会引起CDHA的相反离子反应性，在评估材料的骨诱导潜力时必须仔细考虑这一点。(C)2011 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Solution-mediated surface reactions occur for most calcium phosphate-based biomaterials and may influence cellular response. A reasonable extrapolation of such processes observed in vitro to in vivo performance requires a deep understanding of the underlying mechanisms. We therefore systematically investigated the nature of ion reactivity of calcium-deficient hydroxyapatite (CDHA) by exposing it for different periods of time to standard cell culture media of different chemical composition (DMEM and McCoy medium, with and without osteogenic supplements and serum proteins). Kinetic ion interaction studies of principal extracellular ions revealed non-linear sorption of Ca2+ (similar to 50% sorption) and K+ (similar to 8%) as well as acidification of all media during initial contact with CDHA (48 h). Interestingly, inorganic phosphorus (P-i) was sorbed from McCoy medium (similar to 50%) or when using osteogenic media containing beta-glycerophosphate, but not from DMEM medium. Non-linear sorption data could be perfectly described by pseudo-first-order and pseudo-second-order sorption models. At longer contact time (21 days), and with frequent renewal of culture medium, sorption of Ca2+ remained constant throughout the experiment, while sorption of P-i gradually decreased in McCoy medium. In great contrast. CDHA began to release P-i slowly with time when using DMEM medium. Infrared spectra showed that CDHA exposed to culture media had a carbonated surface chemistry, suggesting that carbonate plays a key role in the ion reactivity of CDHA. Our data show that different compositions of the aqueous environment may provoke opposite ion reactivity of CDHA, and this must be carefully considered when evaluating the osteoinductive potential of the material. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.