Cell separation using tilted-angle standing surface acoustic waves
Cell separation using tilted-angle standing surface acoustic waves
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DOI：
10.1073/pnas.1413325111
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发表时间：
2014-09-09
影响因子：
11.1
通讯作者：
Huang, Tony Jun
中科院分区：
文献类型：
--
作者：
Ding, Xiaoyun;Peng, Zhangli;Huang, Tony Jun
细胞分离是研究细胞特性、疾病诊断和治疗的关键过程。通过声波进行的细胞分选提供了一种基于细胞的大小和物理特性以无标记、非接触和生物相容性方式分离细胞的方法。然而，目前可用的基于声学的方法的分离灵敏度和效率是有限的，从而限制了它们在研究和健康诊断中的广泛应用。在这项工作中，我们引入了一个独特的配置倾斜角驻波表面声波（taSSAW），这是在一个最佳设计的倾斜在微流体通道中的流动方向。我们证明，这种设计显着提高了声学分离技术的效率和灵敏度。为了优化我们的器件设计，我们对细胞轨迹进行了系统的模拟，与实验结果密切匹配。使用数值优化设计的taSSAW，我们成功地分离2-和10-μ m直径的聚苯乙烯珠的分离效率类似于99%，并分离7.3-和9.9-μ m-聚苯乙烯珠的效率类似于97%。我们说明，taSSAW是能够有效地分离颗粒细胞大致相同的大小和密度，但不同的压缩性。最后，我们证明了本技术的生物-生物医学应用的有效性，通过分选MCF-7人乳腺癌细胞从非恶性白细胞，同时保持分离的细胞的完整性。因此，这里介绍的方法为分离循环肿瘤细胞和无标记细胞分离提供了一种独特的途径，在生物研究、疾病诊断和临床实践中具有潜在的应用。
Separation of cells is a critical process for studying cell properties, disease diagnostics, and therapeutics. Cell sorting by acoustic waves offers a means to separate cells on the basis of their size and physical properties in a label-free, contactless, and biocompatible manner. The separation sensitivity and efficiency of currently available acoustic-based approaches, however, are limited, thereby restricting their widespread application in research and health diagnostics. In this work, we introduce a unique configuration of tilted-angle standing surface acoustic waves (taSSAW), which are oriented at an optimally designed inclination to the flow direction in the microfluidic channel. We demonstrate that this design significantly improves the efficiency and sensitivity of acoustic separation techniques. To optimize our device design, we carried out systematic simulations of cell trajectories, matching closely with experimental results. Using numerically optimized design of taSSAW, we successfully separated 2- and 10-mu m-diameter polystyrene beads with a separation efficiency of similar to 99%, and separated 7.3- and 9.9-mu m-polystyrene beads with an efficiency of similar to 97%. We illustrate that taSSAW is capable of effectively separating particles-cells of approximately the same size and density but different compressibility. Finally, we demonstrate the effectiveness of the present technique for biological-biomedical applications by sorting MCF-7 human breast cancer cells from nonmalignant leukocytes, while preserving the integrity of the separated cells. The method introduced here thus offers a unique route for separating circulating tumor cells, and for label-free cell separation with potential applications in biological research, disease diagnostics, and clinical practice.