Role of glucose metabolism in acid formation by isolated gastric glands.
Role of glucose metabolism in acid formation by isolated gastric glands.
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葡萄糖代谢在离体胃腺酸形成中的作用。
DOI：
10.1016/0304-4165(82)90136-2
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Owirodu,A
中科院分区：
文献类型：
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作者：
Hersey,SJ;Miller,M;Owirodu,A
研究了兔离体胃腺中葡萄糖为胃酸形成提供能量的作用。广泛使用的糖酵解抑制剂、碘乙酸和碘乙酰胺抑制葡萄糖氧化，并抑制氨基吡啶的成酸、呼吸和积累指标。然而，发现对酸形成的有效抑制涉及一种非特异性机制，而不是糖酵解的简单抑制。另一种方法涉及使用葡萄糖运输抑制剂，根皮素。根皮素阻断葡萄糖氧化并抑制功能反应。通过添加丙酮酸或各种其他可氧化底物，酸的形成很容易恢复。在没有外源性葡萄糖的情况下，乳酸形成的测量表明胃腺含有很少的糖原。添加外部葡萄糖导致乳酸形成增加10倍，组胺和鱼藤酮进一步刺激了这一速度。鱼藤酮还能抑制呼吸和氨基吡啶的积累；然而，抑制并不完全。根皮素处理导致鱼藤酮处理后残余氨基吡啶积累的完全抑制。结果表明，胃腺几乎完全依赖外部底物供应来支持酸的形成；而且，虽然无氧葡萄糖代谢可以维持非常低水平的酸形成，葡萄糖的主要作用是产生丙酮酸等价物，用于随后的氧化。
The role played by glucose in providing energy for acid formation was studied in isolated gastric glands from rabbit. The widely-used inhibitors of glycolysis, iodoacetic acid and iodoacetamide were found to inhibit glucose oxidation as well as the indicators of acid formation, respiration and accumulation of aminopyrine. However, the potent inhibition of acid formation was found to involve a nonspecific mechanism other than the simple inhibition of glycolysis. An alternative approach involved use of the glucose transport inhibitor, phloretin. Phloretin blocked glucose oxidation and also inhibited functional responses. Acid formation was restored easily by the addition of pyruvate or various other oxidizable substrates. Measurement of lactate formation in the absence of exogenous glucose showed that the gastric glands contain very little glycogen. Addition of external glucose resulted in a 10-fold increase in lactate formation and this rate was stimulated further by histamine and rotenone. Rotenone also inhibited both respiration and aminopyrine accumulation; however, the inhibition was not complete. Phloretin treatment resulted in total inhibition of the residual aminopyrine accumulation after rotenone treatment. The results are interpreted to indicate that gastric glands are dependent almost totally on external substrate supply to support acid formation; and, that while anaerobic glucose metabolism can sustain a very low level of acid formation, the major role of glucose is to yield pyruvate equivalents for subsequent oxidation.