Fatty Acid and Peptide Profiles in Plasma Membrane and Membrane Rafts of PUFA Supplemented RAW264.7 Macrophages
Fatty Acid and Peptide Profiles in Plasma Membrane and Membrane Rafts of PUFA Supplemented RAW264.7 Macrophages
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DOI：
10.1371/journal.pone.0024066
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发表时间：
2011-08-24
期刊：
影响因子：
3.7
通讯作者：
Fuhrmann, Herbert
中科院分区：
文献类型：
--
作者：
Schumann, Julia;Leichtle, Alexander;Fuhrmann, Herbert
真核细胞膜具有许多复杂的功能，这些功能对生命至关重要。在这方面，脂质双层的组成和结构是特别重要的。多不饱和脂肪酸可以通过改变膜脂质组成来调节生物膜的物理性质，从而影响许多生理过程，例如在免疫系统中。在这项系统的研究中，我们提出了脂肪酸和肽的细胞膜和膜筏的小鼠巨噬细胞，已补充了饱和脂肪酸以及PUFA从n-3，n-6和n-9家庭。使用脂肪酸组成分析和基于质谱的肽组分析，我们发现来自n-3和n-6家族的PUFA以类似的方式对质膜和膜筏的脂质和蛋白质组成产生影响。此外，我们发现添加的多不饱和脂肪酸的双烯丙基亚甲基的位置的数量之间的关系和质膜的不饱和指数，以及补充细胞的膜筏。关于脂质微区对疾病发展和治疗的拟议意义，我们的研究将有助于实现免疫细胞脂质双层的靶向饮食调节。
The eukaryotic cell membrane possesses numerous complex functions, which are essential for life. At this, the composition and the structure of the lipid bilayer are of particular importance. Polyunsaturated fatty acids may modulate the physical properties of biological membranes via alteration of membrane lipid composition affecting numerous physiological processes, e.g. in the immune system. In this systematic study we present fatty acid and peptide profiles of cell membrane and membrane rafts of murine macrophages that have been supplemented with saturated fatty acids as well as PUFAs from the n-3, the n-6 and the n-9 family. Using fatty acid composition analysis and mass spectrometry-based peptidome profiling we found that PUFAs from both the n-3 and the n-6 family have an impact on lipid and protein composition of plasma membrane and membrane rafts in a similar manner. In addition, we found a relation between the number of bis-allyl-methylene positions of the PUFA added and the unsaturation index of plasma membrane as well as membrane rafts of supplemented cells. With regard to the proposed significance of lipid microdomains for disease development and treatment our study will help to achieve a targeted dietary modulation of immune cell lipid bilayers.