Determination of acetone in breath
Determination of acetone in breath
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DOI：
10.1016/j.aca.2004.12.018
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发表时间：
2005-04
影响因子：
6.2
通讯作者：
N. Teshima;Jianzhong Li;K. Toda;P. Dasgupta
中科院分区：
文献类型：
--
作者：
N. Teshima;Jianzhong Li;K. Toda;P. Dasgupta
A light emitting diode (LED)-based photometric method for the measurement of gaseous acetone in human breath is presented. The detection chemistry is based on the reaction of acetone with alkaline salicylaldehyde to form a colored product, which absorbs in the blue and can be monitored with GaN-based LEDs with emission centered at 465nm. Gaseous acetone in breath is sampled with a porous membrane based diffusion scrubber (DS). The collected sample in the continuously flowing water carrier reacts with the reagent solution. We have used two approaches to collect breath acetone: the use of a face mask and a Mylar balloon as a collection bag. With the face mask approach, the expired air can be measured over long periods without major subject discomfort, balloon collection (5l) permits four measurements from a single fill. The LED-based liquid core waveguide (LCW) absorbance detector utilized sapphire ball lenses to prevent exposure of other optical components to a hot alkaline reagent solution. The high refractive index of the final mixture permitted the use of an inexpensive fluorinated ethylene copolymer (FEP Teflon®) tube as a 10cm long LCW. The limit of detection (S/N=3) is 14ppbv gaseous acetone, and the linear range extends to 1.21ppmv. The concentration range in 11 volunteer subjects ranged from 176 to 518ppbv.
