Correlation between angiotensin-converting enzyme, vascular endothelial growth factor, and matrix metal loproteinase-9 in the vitreous of eyes with diabetic retinopathy
Correlation between angiotensin-converting enzyme, vascular endothelial growth factor, and matrix metal loproteinase-9 in the vitreous of eyes with diabetic retinopathy
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DOI：
10.1016/j.ajo.2005.08.066
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发表时间：
2006-01-01
影响因子：
4.2
通讯作者：
Miyazaki, M
中科院分区：
文献类型：
--
作者：
Ishizaki, E;Takai, S;Miyazaki, M
目的：探讨血管紧张素转换酶（ACE）与血管生成因子、血管内皮生长因子（VEGF）和基质金属蛋白酶（MMP） 9在增殖性糖尿病视网膜病变（PDR）眼玻璃体中的作用。设计：观察性病例系列。方法：用ACE合成底物测定玻璃体血管紧张素转化酶活性。采用酶联免疫吸附法测定VEGF和MMP-9浓度。结果：PDR组玻璃体ACE活性（1 ~ 4 +/- 1.3 mU/ml）明显高于黄斑孔组（0.22 +/- 0.11 mU/ml）。PDR组玻璃体中VEGF浓度（1067 ~ 1076 pg/ml）明显高于黄斑孔组（34 +/- 5.5 pg/ml）。PDR眼的玻璃体MMP-9浓度（7.5 +/- 3.8 ng/ml）明显高于黄斑孔眼（4.2 +/- 1.4 ng/ml）。在PDR眼玻璃体中，ACE与VEGF、MMP-9均有显著相关性（P < 0.01）。结论：PDR眼玻璃体血管紧张素转换酶与血管生成因子VEGF、MMP，9有显著相关性。
PURPOSE: To investigate the involvement of angiotensin-converting enzyme (ACE) with angiogenic factors, vascular endothelial growth factor (VEGF), and matrix metalloproteinase (MMP),9 in the vitreous of eyes with proliferative diabetic retinopathy (PDR).DESIGN: Observational case series.METHODS: Angiotensin-conerting enzyme activity in the vitreous was measured by using a synthetic substrate for ACE. VEGF and MMP-9 concentrations were deter, mined by enzyme,linked immunosorbent assay.RESULTS: Vitreous ACE activity was significantly higher in eyes with PDR (1-4 +/- 1.3 mU/ml) than in eyes with macular holes (0.22 +/- 0.11 mU/ml). Vitreous VEGF concentration in the eyes with PDR (1067 1076 pg/ml) was significantly higher than in eyes with macular holes (34 +/- 5.5 pg/ml). Vitreous MMP-9 concentration was also significantly higher in eyes with PDR (7.5 +/- 3.8 ng/ml) than in eyes with macular holes (4.2 +/- 1.4 ng/ml). Significant correlations between ACE and VEGF (P < .01) and between ACE and MMP-9 (P < .01) were observed in the vitreous of eyes with PDR. 0CONCLUSIONS: Angiotensin-converting enzyme was significantly correlated with angiogenic factors, VEGF and MMP,9, in the vitreous of eyes with PDR.