Development of bio-based fine chemical production through synthetic bioengineering.
Development of bio-based fine chemical production through synthetic bioengineering.
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DOI：
10.1186/s12934-014-0173-5
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发表时间：
2014-12-14
影响因子：
6.4
通讯作者：
Kondo A
中科院分区：
文献类型：
--
作者：
Hara KY;Araki M;Okai N;Wakai S;Hasunuma T;Kondo A
具有生理活性的精细化学品，如药品、化妆品、营养补充剂、调味剂以及食品、饲料和肥料的添加剂，通过酶或通过微生物发酵生产。催化目标反应的酶的鉴定使得所需精细化学品的酶促合成成为可能。然后将编码这些酶的基因引入合适的微生物宿主中，所述微生物宿主与廉价的、天然丰富的碳源和其他营养物一起培养。代谢工程创造了高效的微生物细胞工厂，以更高的产量生产化学品。分子遗传技术，然后用于优化代谢途径的遗传和代谢特征良好的主机。合成生物工程代表了一种新型方法，采用计算机模拟和代谢分析相结合来设计适合在宿主菌株中大规模生产目标化学品的人工代谢途径。在本综述中，我们总结了最近的研究，生物基精细化工生产和评估的潜力，进一步提高其生产力的合成生物工程。
Fine chemicals that are physiologically active, such as pharmaceuticals, cosmetics, nutritional supplements, flavoring agents as well as additives for foods, feed, and fertilizer are produced by enzymatically or through microbial fermentation. The identification of enzymes that catalyze the target reaction makes possible the enzymatic synthesis of the desired fine chemical. The genes encoding these enzymes are then introduced into suitable microbial hosts that are cultured with inexpensive, naturally abundant carbon sources, and other nutrients. Metabolic engineering create efficient microbial cell factories for producing chemicals at higher yields. Molecular genetic techniques are then used to optimize metabolic pathways of genetically and metabolically well-characterized hosts. Synthetic bioengineering represents a novel approach to employ a combination of computer simulation and metabolic analysis to design artificial metabolic pathways suitable for mass production of target chemicals in host strains. In the present review, we summarize recent studies on bio-based fine chemical production and assess the potential of synthetic bioengineering for further improving their productivity.
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影响因子：
8.4
作者：
Farhi, Moran;Marhevka, Elena;Vainstein, Alexander
通讯作者：
Vainstein, Alexander
DOI：
10.1126/science.1191652
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ajikumar PK;Xiao WH;Tyo KE;Wang Y;Simeon F;Leonard E;Mucha O;Phon TH;Pfeifer B;Stephanopoulos G
通讯作者：
Stephanopoulos G
影响因子：
11.4
作者：
Aikawa, Shimpei;Izumi, Yoshihiro;Kondo, Akihiko
通讯作者：
Kondo, Akihiko
影响因子：
3.8
作者：
Dai, Zhubo;Liu, Yi;Zhang, Xueli
通讯作者：
Zhang, Xueli
影响因子：
14.8
作者：
Bastard, Karine;Smith, Adam Alexander Thil;Salanoubat, Marcel
通讯作者：
Salanoubat, Marcel