Immunolocalization of Tenascin-C in Vitiligo
Immunolocalization of Tenascin-C in Vitiligo
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DOI：
10.1097/pai.0b013e318246c793
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发表时间：
2012-10-01
影响因子：
1.6
通讯作者：
Elbana, Rania
中科院分区：
文献类型：
--
作者：
Abdou, Asmaa Gaber;Maraee, Alaa Hassan;Elbana, Rania
黑素细胞因黏附缺陷而消失是解释白癜风的公认理论之一。Tenascin-C是一种大的细胞外基质糖蛋白，被认为可以抑制黑素细胞与纤维连接蛋白的黏附。本研究旨在通过免疫组织化学方法研究Tenascin-C在白癜风皮肤和正常色素性皮肤中的表达模式。本研究对30例白癜风患者的皮损及皮损周围皮肤和10例健康志愿者的正常皮肤进行了皮肤活检。白斑皮肤可见角质形成细胞空泡化、基底膜增厚、真皮炎性改变等组织病理学改变。Tenascin-C在正常皮肤活检的基底层角质形成细胞中呈弱阳性表达，但在真皮中不染色，而白癜风皮肤中Tenascin-C在大多数病例中表达(93.3%)，在乳头状真皮、真皮和两者中均有表达。表皮中Tenascin-C的弥漫性表达与更多的色素丢失和乳头状真皮中Tenascin-C的持续染色相关，与进行性白癜风相关。通过白癜风疾病活动性评分评估，Tenascin-C的强烈表达与疾病的进展相关。从这项研究来看，Tenascin-C在白癜风的真皮、表皮和两者中都高度表达，是白癜风的继发性事件。角质形成细胞是白癜风中Tenascin-C的来源之一，表皮弥漫性表达Tenascin-C可能会导致更多的黑素细胞和黑色素的丢失。白斑性皮损中Tenascin-C的真皮表达可能比表皮表达与疾病的关系更密切，因为这种模式仅见于白斑性皮损，而在正常和皮损周围皮肤中完全不存在。
The disappearance of melanocytes because of defective adhesion is one of the accepted theories to explain vitiligo. Tenascin-C is a large, extracellular matrix glycoprotein that is thought to inhibit adhesion of melanocytes to fibronectin. The current study aimed to evaluate the pattern of tenascin-C expression in vitiligenous skin compared with normal pigmented skin by means of immunohistochemistry. The study was carried out on skin biopsies from lesional and perilesional skin of 30 patients with vitiligo and on normal skin of 10 healthy volunteers. Several histopathologic changes were observed in vitiliginous skin such as keratinocyte vacuolization, a thickened basement membrane, and dermal inflammatory changes. Tenascin-C was expressed in keratinocytes of the basal epidermal layer of normal skin biopsies at a mild intensity but it did not stain the dermis, whereas vitiligenous skin showed tenascin-C expression in most cases (93.3%), in the papillary dermis, epidermis, and in both. Diffuse epidermal expression of tenascin-C correlated with more loss of pigment and continuous staining of tenascin-C in the papillary dermis correlated with progressive forms of vitiligo. Intense tenascin-C expression was associated with a more progressive course of the disease assessed by the vitiligo disease activity score. From this study, tenascin-C is highly expressed in the dermis, epidermis, and both of vitiligo as a secondary event for the disease. Keratinocyte is a source of tenascin-C in vitiligo, and diffuse epidermal expression of tenascin-C may induce more loss of melanocytes and melanin pigment. Dermal expression of tenascin-C in the vitiligenous lesion may be linked to the disease more than epidermal expression, because this pattern is only seen in a vitiligenous lesion and it is completely absent in normal and perilesional skin.