Effect of vibration on osteoblastic and osteoclastic activities: Analysis of bone metabolism using goldfish scale as a model for bone
Effect of vibration on osteoblastic and osteoclastic activities: Analysis of bone metabolism using goldfish scale as a model for bone
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DOI：
10.1016/j.asr.2007.04.104
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发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Hattori, A.
中科院分区：
文献类型：
--
作者：
Suzuki, N.;Kitamura, K.;Hattori, A.
在空间飞行期间的骨代谢活动以及尾部悬吊的后肢卸载中，在体内研究中报告了不一致的结果。骨基质在对物理应力的反应中起重要作用。然而，目前还没有合适的成骨细胞和破骨细胞包括骨基质的体外共培养体系。另一方面，鱼鳞是一种钙化组织，含有成骨细胞，破骨细胞和骨基质，所有这些都与人类骨骼中发现的相似。最近，我们开发了一种新的体外模型系统，使用金鱼鳞片。该系统可以分别以抗酒石酸酸性磷酸酶和碱性磷酸酶为标志物检测破骨细胞和成骨细胞的活性，并准确分析成骨细胞和成骨细胞之间的相互关系。使用该系统，我们分析了G载荷装置振动产生不同加速度（0.5-、1-、2-、4-和6-G）下的骨代谢。加载5和10 min后，将鳞片孵育6和24 h。然后测量成骨细胞和骨细胞活性。成骨细胞活性随1-G ~ 6-G加速度的增加而逐渐增强。此外，ER mRNA表达在6-G加速度下最高。另一方面，在低加速度（0.5-和1-G）下孵育24小时，骨细胞活性降低。这一变化与TRAP mRNA表达一致。在2-G加速度下，对骨细胞活性的抑制强度最大。4-和6-G加速度下的抑制作用强度低于2-G加速度。在我们的共培养系统中，成骨细胞和破骨细胞的规模敏感地回应了几个度的加速度。因此，我们坚信，我们的体外共培养系统是有用的，在加载或卸载下的骨代谢分析。(c)2007年空间研委会。由爱思唯尔有限公司出版。保留所有权利。
In osteoclastic activity during space flight as well as hind limb unloading by tail suspension, inconsistent results have been reported in an in vivo study. The bone matrix plays an important role in the response to physical stress. However, there is no suitable in vitro co-culture system of osteoblasts and osteoclasts including bone matrix. On the other hand, fish scale is a calcified tissue that contains osteoblasts, osteoclasts, and bone matrix, all of which are similar to those found in human bones. Recently, we developed a new in vitro model system using goldfish scale. This system can detect the activities of osteoclasts and osteoblasts with tartrate-resistant acid phosphatase and alkaline phosphatase as the respective markers and precisely analyze the co-relationship between osteoblasts and osteoclasts. Using this system, we analyzed the bone metabolism under various degrees of acceleration (0.5-, 1-, 2-, 4-, and 6-G) by vibration with a G-load apparatus. After loading for 5 and 10 min, the scales were incubated for 6 and 24 h. The osteoblastic and osteoclastic activities were then measured. The osteoblastic activities gradually increased corresponding to 1-G to 6-G acceleration. In addition, ER mRNA expression was the highest under 6-G acceleration. On the other hand, the osteoclastic activity decreased at 24 h of incubation under low acceleration (0.5- and 1-G). This change coincided with TRAP mRNA expression. Under 2-G acceleration, the strength of suppression in osteoclastic activity was the highest. The strength of the inhibitory action under 4- and 6-G acceleration was lower than that under 2-G acceleration. In our co-culture system, osteoblasts and osteoclasts in the scale sensitively responded to several degrees of acceleration. Therefore, we strongly believe that our in vitro co-culture system is useful for the analysis of bone metabolism under loading or unloading. (c) 2007 COSPAR. Published by Elsevier Ltd. All rights reserved.