Light-arousal and circadian photoreception circuits intersect at the large PDF cells of the Drosophila brain
Light-arousal and circadian photoreception circuits intersect at the large PDF cells of the Drosophila brain
复制标题
DOI：
10.1073/pnas.0809577105
复制
发表时间：
2008-12-16
影响因子：
11.1
通讯作者：
Rosbash, Michael
中科院分区：
文献类型：
--
作者：
Shang, Yuhua;Griffith, Leslie C.;Rosbash, Michael
调节睡眠和觉醒的神经回路及其与昼夜节律回路的整合仍不清楚，特别是在果蝇中。这个问题与感光问题相交叉，因为光既是昼夜动物的唤醒信号，也是生物钟的引导信号。为了识别与光介导的唤醒和昼夜节律相移相关的神经元和电路，我们开发了将行为与果蝇中脑内单细胞类型分辨率联系起来的遗传技术。我们重点关注果蝇昼夜节律大脑网络中 10 个包含 PDF 的大型腹侧外侧神经元 (I-LNv) 的未知功能，并在此表明这些细胞在光依赖性唤醒中发挥作用。它们对于深夜（黎明）的相移也很重要，这表明昼夜节律光响应是一种网络特性，因此不是细胞自主的。数据进一步表明，光诱导唤醒和昼夜节律光诱导的电路在 I-LNv 处相交，然后分离。
The neural circuits that regulate sleep and arousal as well as their integration with circadian circuits remain unclear, especially in Drosophila. This issue intersects with that of photoreception, because light is both an arousal signal in diurnal animals and an entraining signal for the circadian clock. To identify neurons and circuits relevant to light-mediated arousal as well as circadian phase-shifting, we developed genetic techniques that link behavior to single cell-type resolution within the Drosophila central brain. We focused on the unknown function of the 10 PDF-containing large ventral lateral neurons (I-LNvs) of the Drosophila circadian brain network and show here that these cells function in light-dependent arousal. They also are important for phase shifting in the late-night (dawn), indicating that the circadian photoresponse is a network property and therefore non-cell-autonomous. The data further indicate that the circuits underlying light-induced arousal and circadian photoentrainment intersect at the I-LNvs and then segregate.