SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells.
SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells.
复制标题
DOI：
10.1016/s1673-8527(09)60042-4
复制
发表时间：
2010-04
影响因子：
5.9
通讯作者：
Bishop, Colin E.
中科院分区：
文献类型：
--
作者：
Pan, Chuanying;Hicks, Amy;Guan, Xuan;Chen, Hong;Bishop, Colin E.
诱导多能干细胞（iPS）可以通过细胞重编程从人类体细胞衍生。这项技术为组织修复、药物发现和研究人类疾病的分子基础提供了无争议的治疗细胞的潜在来源。通常，小鼠胚胎成纤维细胞（MEF）在iPS系的初始衍生中用作饲养层。本研究的目的是确定SNL成纤维细胞是否可用于支持使用慢病毒表达的重编程因子从体细胞重编程的人iPS细胞的生长。在我们的研究中，iPS细胞表达常见的多能性标志物，显示人胚胎干细胞（hESC）形态和NANOG和OCT 4的非甲基化启动子。这些数据表明SNL饲养细胞可以支持人iPS细胞的衍生和维持。
Induced pluripotent stem (iPS) cells can be derived from human somatic cells by cellular reprogramming. This technology provides a potential source of non-controversial therapeutic cells for tissue repair, drug discovery, and opportunities for studying the molecular basis of human disease. Normally, mouse embryonic fibroblasts (MEFs) are used as feeder layers in the initial derivation of iPS lines. The purpose of this study was to determine whether SNL fibroblasts can be used to support the growth of human iPS cells reprogrammed from somatic cells using lentiviral expressed reprogramming factors. In our study, iPS cells expressed common pluripotency markers, displayed human embryonic stem cells (hESCs) morphology and unmethylated promoters of NANOG and OCT4. These data demonstrate that SNL feeder cells can support the derivation and maintenance of human iPS cells.