Ca2+ Channel to Synaptic Vesicle Distance Accounts for the Readily Releasable Pool Kinetics at a Functionally Mature Auditory Synapse
Ca2+ Channel to Synaptic Vesicle Distance Accounts for the Readily Releasable Pool Kinetics at a Functionally Mature Auditory Synapse
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DOI：
10.1523/jneurosci.2753-14.2015
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发表时间：
2015-02-04
影响因子：
5.3
通讯作者：
Young, Samuel M., Jr.
中科院分区：
文献类型：
--
作者：
Chen, Zuxin;Das, Brati;Young, Samuel M., Jr.
准确调节Hold区的突触小泡(SV)释放是听觉加工的关键。在听前保持的花萼，同步和非同步释放是由易释放池(RRP)内的快速和缓慢释放的SVS介导的。然而，缝合后的花萼具有显著不同的释放特性。RRP特性的发育变化是否有助于在花瓣愈合后发现加速释放时间进程尚不清楚。为了研究这些问题，我们进行了成对的膜片钳记录、去卷积分析和数值模拟出生后16-19天的小鼠肾盏的缓冲的钙离子扩散和SV的释放，因为它们的释放特性类似于Hold的成熟的肾盏。我们发现P16-P19 Calyx RRP由两个池组成：一个FAST池(tau
Precise regulation of synaptic vesicle (SV) release at the calyx of Held is critical for auditory processing. At the prehearing calyx of Held, synchronous and asynchronous release is mediated by fast and slow releasing SVs within the readily releasable pool (RRP). However, the posthearing calyx has dramatically different release properties. Whether developmental alterations in RRP properties contribute to the accelerated release time course found in posthearing calyces is not known. To study these questions, we performed paired patch-clamp recordings, deconvolution analysis, and numerical simulations of buffered Ca2+ diffusion and SV release in postnatal day (P) 16-19 mouse calyces, as their release properties resemble mature calyces of Held. We found the P16-P19 calyx RRP consists of two pools: a fast pool (tau