A molecular revolution in the study of intestinal microflora
A molecular revolution in the study of intestinal microflora
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DOI：
10.1136/gut.2005.081695
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发表时间：
2006-02-01
期刊：
影响因子：
24.5
通讯作者：
Furrie, E
中科院分区：
文献类型：
--
作者：
Furrie, E
结肠的细菌定植者包括数百种生活在复杂生态系统中的细菌。直到最近，对这种复杂生态系统的研究一直是通过传统的培养技术和生物化学方法来识别生物。研究生态微生物群落的分子技术的发展为微生物学家提供了大量研究人类肠道菌群的新技术。宏基因组学是一门研究生物多样性的科学，它结合了分子生物学和遗传学的应用，从复杂的微生物环境中识别和鉴定遗传物质。宏基因组学和随后使用分子技术对每个鉴定的物种进行定量的组合允许相对快速地分析人类健康和疾病中的整个细菌群体。
Bacterial colonisers of the colon comprise several hundred bacterial species that live in a complex ecosystem. Study of this complex ecosystem has been carried out, until recently, by traditional culture techniques with biochemical methods to identify organisms. The development of molecular techniques to investigate ecological microbial communities has provided the microbiologist with a vast array of new techniques to investigate human intestinal microflora. Metagenomics, the science of biological diversity, combines the use of molecular biology and genetics to identify and characterise genetic material from complex microbial environments. The combination of metagenomics and subsequent quantitation of each identified species using molecular techniques allows the relatively rapid analysis of whole bacterial populations in human health and disease.