ANALYSIS OF THE COLLAGEN AND TOTAL SOLUBLE NITROGEN-CONTENT OF ISINGLASS FININGS BY POLARIMETRY
ANALYSIS OF THE COLLAGEN AND TOTAL SOLUBLE NITROGEN-CONTENT OF ISINGLASS FININGS BY POLARIMETRY
复制标题
DOI：
10.1002/j.2050-0416.1994.tb00831.x
复制
发表时间：
1994-09-01
影响因子：
2.6
通讯作者：
LYDDIATT, A
中科院分区：
文献类型：
--
作者：
LEATHER, RV;SISK, M;LYDDIATT, A
通过对单一鱼鳔鱼鳍制品的胶原蛋白和明胶的分析表明，每种制剂的特定旋光度在给定波长的入射光下是一致的，并且与鱼鳔鱼鳍源无关。这些特定旋转值与鱼胶制剂的特定旋转测量相结合，可以直接估计胶原蛋白含量。此外，鱼胶制剂的热变性和所得明胶的总可溶性氮含量的偏振测定为鱼胶细粒的总可溶性氮含量的凯氏定氮法测定提供了一种快速方便的替代方法。通过偏振法测量观察到的鱼胶制剂的旋转和相同材料的热变性等分液的总可溶性氮含量，可以在40分钟内分析胶原蛋白和总可溶性蛋白含量，而传统的基于鱼胶制剂透析的质量控制程序需要24小时。
Analysis of collagen and gelatin derived from single type fishbladder preparations of isinglass finings demonstrated that the specific optical rotation of each preparation was consistent for a given wavelength of incident light and was independent of the isinglass source. These specific rotation values in conjunction with measurement of the specific rotation of an isinglass preparation enabled the direct estimation of collagen content. Furthermore, thermal denaturation of isinglass preparations and polarimetric determination of the total soluble nitrogen content of the resulting gelatin provides a rapid and convenient alternative to Kjeldahl determination of the total soluble nitrogen content of isinglass finings. Measurement of the observed rotation of isinglass preparations and the total soluble nitrogen content of thermally denaturated aliquots of the same material by polarimetry enabled the analysis of collagen and total soluble protein content within 40 minutes compared to 24 hours for conventional quality control procedures based on dialysis of isinglass preparations.