Carboxymethyl cellulose-hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications
Carboxymethyl cellulose-hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications
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DOI：
10.1002/jbm.a.34810
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发表时间：
2014-05-01
影响因子：
4.9
通讯作者：
Barbucci, Rolando
中科院分区：
文献类型：
--
作者：
Pasqui, Daniela;Torricelli, Paola;Barbucci, Rolando
天然骨是一种复杂的无机 - 有机纳米复合材料，其中羟基磷灰石（HA）纳米晶体和胶原纤维在多个长度尺度上有序排列成层级结构。在这项工作中，我们报道了一种新型杂化材料（CMC - HA），它是将HA混入羧甲基纤维素（CMC）基水凝胶中。将HA纳米晶体插入水凝胶基质的策略是使冷冻干燥的水凝胶在含有HA微晶的溶液中溶胀。通过傅里叶变换红外光谱、流变学测量和场发射扫描电子显微镜（FESEM）从物理化学和形态学角度对复合CMC - HA水凝胶进行了表征。在水或氯化钠溶液中未检测到HA的释放。通过飞行时间二次离子质谱（ToF - SIMS）和FESEM确定了HA晶体在水凝胶表面和内部的分布。使用成骨细胞MG63系测试了CMC - HA水凝胶的生物学性能，并与不含HA的CMC基水凝胶进行了比较。对成骨细胞标志物和基因表达的评估表明，在CMC水凝胶中添加HA可增强细胞增殖和代谢活性，并促进矿化细胞外基质的产生。（c）2013威利期刊公司。《生物医学材料研究杂志A部分》：102A：1568 - 1579，2014。
Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales. In this work, we reported a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel. The strategy for inserting HA nanocrystals within the hydrogel matrix consists of making the freeze-dried hydrogel to swell in a solution containing HA microcrystals. The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM). No release of HA was measured in water or NaCl solution. The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM. The biological performance of CMC-HA hydrogel were tested by using osteoblast MG63 line and compared with a CMC-based hydrogel without HA. The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1568-1579, 2014.